Cloning And Eepression Analysis Of Several Flora Genes In Carya Cathayensis Sarg.

Carya cathayensis Sarg. is endemic to Chinese famous dried fruit and Woody oil plant, with high economic value. Carya cathayensis is a hermaphrodite outcrossing woody plants, have problems such as a long time ahead of maturation and male and female flower development time inconsistency which directly affect it's production. This paper will probe into Carya cathayensis flowering mechanism and the mechanism of sex floral organs, for fundamentally improving the Carya cathayensis production or realization of precocious and dwarf provide the scientific basis. The paper will use homology cloning and genome walking techniques clone a number of flower-related genes in Carya cathayensis, use Real-Time RT PCR technology and RNA in situ hybridization analysis gene expression in different flowering time and different issues of female flower bud. The main conclusions are as follows:FT, AG and AP1 homologous gene fragments have been cloned in Carya cathayensis, named CcFT, CcAG and CcAP1. Homology analysis between CcFT, CcAG and CcAP1 gene sequence and the corresponding genes of other plants showed that they have a high homology,this structural similarity suggested that they have similar function.Expression analysis of the CcAP1,we found that its expressing during the female flower bud formation did not show obvious rule.Most times the expression were low or almost no expression. Related studies have shown that AP1 may related to development of the sepals and petals. Carya cathayensis female flower lack of perianth,that may result in low expression of CcAP1.Carya cathayensis CcLFY expresses during the whole process of the formation of the female flower bud , but very different in different times. Real-Time RT PCR results showed that, CcLFY expresses significantly higher in 3 months early than all samples following, expression of each late sample is relatively low. Also found that the expression of CcLFY affected by temperature, low temperature will reduce the expression of CcLFY to some extent.In situ hybridization results showed that, CcLFY hybridization signal during female flower bud primordia is not very strong, with the growth cone in the top inflorescence primordia gradually flattened, hybridization signal was enhanced. CcLFY hybridization signals also detected during the formation of small flowers, small bracts process. These results suggest that CcLFY may plays an important role in female flower bud formation in Carya cathayensis, and may also affect the formation of organ differentiation.