Study On The Immune Adjustment Functions Of Ultramicro-bacteria'Polysaccharides To Animal

Polysaccharide exists widely in the cell wall of plant, animal, microorganism and epiphyte. As a kind of biologic substance having the action of immune regulation, it can enhance the function of both the nonspecific immunity and the specific immunity.The extracting conditions of polysaccharide from ultramicro-bacteria Glaciecola polaris by water and ultrasonic treatment were studied in this paper. Effects on sample to water, extracting temperature and extracting time for the polysaccharide yield, mucosity and surface tension were observed in normal extraction. The results showed that there were significant differences between polysaccharide yield in different extracting time (p<0.05 ). The optimum extracting conditions were as follow: the rate of sample to water (w: v), the extracting temperature and the extracting time were 1:15, 100℃and 4h~6h respectively. The highest polysaccharide yield could be reach to 8.08%. Ultrasonic treatment was proved to be an efficient method to enhance the rate of polysaccharide yield, decrease the mucosity and the surface tension of solution, which can shorten the extracting time and reduce the thickness resistance to be prone to separating so that more yield can be gain. 100W ultrasonic power and 10-15min ultrasonic treatment would be the optimum conditions extracted by water for 4h, and the t polysaccharide yield probably could be reach to 8.82%.An experiment was conducted to study the polysaccharide extracted from ultramicro-bacteria Glaciecola polaris on tumor immunization, designed by feeding plamasol to observe the inhibiting tumor trial and using MTT to assay macrophage mediated cytotoxicity .The activities of SOD were observed to determine the effect of antiagting. The results showed that polysaccharide had evident inhibitive effect on S180 tumor growth (P<0.05-0.01). Significant increase (P<0.01) was observed at the prolong longetive rate on them. The induction of nitric oxide in murtur macrophages and macrophage-mediated cytotoxicity in polysaccharide group and germ powder were significantly higher than those of control group (P<0.05～0.01), and high concentration (100uL) is better than less. These observations suggest that polysaccharide could be susceptible macrophage to increase NO production and improve the efficiency of killing tumor cells. Significant increase (P<0.01～0.05) was also observed at the SOD activities between treatments and control, which indicated that the polysaccharide could increase humoral immunity.Effect of polysaccharide(PRK), extracted from ultramicro-bacteria Glaciecola polaris was in vitro investigated in protection of oxidatively- injured mouse macrophages stressed by tert-butyl hydroperoxide (tbOOH) or by oxidatively modified low-density lipoprotein (Ox-LDL). The results showed that PSK treatments to protect macrophages from oxidative injury would be effective and macrophage colony-stimulating factor (M-CSF) exhibited some similar effects. The survival rate of PSK-treated mouse peritoneal macrophages or rhM-CSF-pretreated macrophages cells was significantly higher than that of non-PSK-treated or non-M-CSF-pretreated control cells (p<0.01). Peritoneal macrophages-conditioned medium (PM-CM) obtained from PSK-treated mouse peritoneal macrophages exhibited significantly higher colony-stimulating activity than the conditioned medium of non-PSK-treated cells (p < 0.05) and PSK could significantly increase M-CSF mRNA content in mouse peritoneal macrophages (p<0.05). It was speculated that both M-CSF and PSK could protect macrophages from oxidative injury. PSK-treated macrophages could release more M-CSF and M-CSF could alleviate the Ox-LDL-induced or tbOOH -induced injury to mouse peritoneal macrophages. We proposed that effects of PSK were associated with its capability of inducing M-CSF expression.Polysaccharides extracted from ultramicro-bacteria Glaciecola polaris on immune and anti-stress ability in Chinese soft-shelled turtle stressed by pH were investigated. The results showed that the significant increases (P<0.01) were observed at the phagocytic rate of blood cells, serum bacteriolytic activity and bactericidal bacteriolytic activity in polysaccharide groups. We proposed that effects of PSK might regulate the incretion disturbed by acid-stress and enhance the function of immunity.Effect of polysaccharide on promoting the growth capability and enhancing the function of immunity in Chinese soft-shelled turtle (50-60g) is studied by supplying 0.05 %,0.1 % polysaccharide level, or 0.05 %, 0.1 % germ powder in diets. After 8 weeks, a significant increases (P<0.05-0.01) are observed at the growth efficiency, immune tissues index, SOD activity and feed efficiency in polysaccharides groups. No significant effect on serum bacteriolytic activity is observed ( P>0.05) between trial groups and control group. We proposed that the optimum polysaccharide accession should be 0.05 % in diets.