The Effect Of Epiphytic Lactic Acid Bacteria On Gramineous Grass Silage Fermentation Characteristics And Dairy Cows Performance

The object is that selected epiphytic Lactic acid bacteria(LAB) from gramineous grass Elymus Excelsus and Elymus sirbiricus, Isolated, identified and selected excellent LAB strains. Then the strain or strains were applied as inoculants on grass, investigated the effect on fermentation characteristics. Made field silage applying the strains, detected dairy performance. It is a valuable study how to improve graminouse grass feed value and feeding effect.Fifteen LAB strains were isolated and selected 2 excellent strains from it via morphologic, physiology, biochemistry and carbohydrate fermentation, They were Lactobacillus plantarum and Enterococcus mundtti, they possessed some good quality, such as, rapidly fermenting, extensive growth in different temperature, facilely utilized carbohydrate to form lactic acid, homofermentation LAB. Their multiplication was rapid, the highest alive colony forming unit was about 1010 cfu/ml broth.A study was conducted to investigate the effect of applying Lactobacillus Plantarum and Enterococcus Mundtti on fermentation characteristics of mixed gramineous grass Elymus Excelsus and Elymus sirbiricus. There were four treatments as followed: the control (no additives) (Con), the E.Mundtii (Em), the L.Plantarum (Lp) and the E.Mundtii added in L.Plantarum (EL). Each treatment was carried out 8 repetitions. The results showed: 1) all LAB inoculants could significantly (P<0.05) reduce silage pH value compared with the control. 2) All LAB inoculants could evidently (P<0.01) increase the content of Lactic acid (LA) and the ratio of Lactic acid to acetic acid (AA) in silage, especially the EL; and distinctly (P<0.05) decrease the content of propionic acid (PA) and butyric acid (BA), compared with Con. 3) The silage inoculated with EL had the lowest concentration of ammonia-N (20.85umol/gFM). 4) All LAB inoculants could reduce dry matter loss , Em,Lp,EL, respectively , 0.98,1.32,0.6%. As a result, applying LAB can indeed improve silage fermentation quality. EL treatment was the best one.A study was conducted to investigate the effect of applying Lactobacillus Plantarum and Enterococcus Mundtti on fermentation characteristics of Bromus inermis of field silage, and the effect on dairy cows performance. As a result, supplement Bromus inermis silage in diets, silage treatment increased evidently (p<0.05) dry matter intake (DMI) compared with hay, respectively, 14.28 and 16.71kg/d. Silage treatment promoted milk yield 0.52kg/d (p<0.1), increased distinctly milk dry matter (p<0.05) content and milk fat (p<0.05) content. But there were not obviously change about dry matter removed fat (p>0.05), lactose (p>0.05) and casein (p>0.05). Furthermore,β-Carotene Levels in silage and hay could supply sufficiently vitamin A in dairy cows diets, respectively, 43.87ug/ml and 42.20ug/ml. Feeding inoculated silage had a trend to increase LAB (p<0.1) and Enteroccus (p<0.1), decrease enterobacteria (p<0.1), and have no effect on the common bacilli (p>0.05).