Identification Of Molecular Markers Linked To Cladosporium Fulvum Resistance Genes Cf₁₁ And Cf₁₉ In Tomato

The study collected and identified tomato leaf mold pathogen strains in main tomato growth area of northeast three provinces.Based on the genetic analysis of resistance to Cladosporium fulvum,AFLP and SSR markers that linked to the resistance gene Cf₁₁ and Cf₁₉ were identified.At the same time,tomato germplasm resistant to Cladosporium fulvum were screened with inculation and mocular markers on AFLP.1.The article analysed and identified leaf mold pathogen strains differentiation.The results indicated that physiological race 1.2,race2.3,race 1.2.3,race1.2.4 and race1.2.3.4 were found.The race 1.2.3.4 and race 1.2.4 was dominant race.Race 1.2.3.4 occupied 57.75%,and race 1.2.3 occupied 20.1%.2.Resistance to race 1.2.3.4 of Cladosporium fulvum was found to due to a single dominant gene through analyzing resistance behavior of the cross HN19(susceptible)×NHN43 (resistable).The results indicated that the diffence of two parents resistanc and susceptibility is obvious,the male parent HN43 shows resistance and the female parent HN19 shows susceptibility.The ratio of number of resistance and susceptibility on F2 is 3.08:1.00,which accord with the ratio of 3:1,which accord with the ratio of 1:1.The results shows resistance to Cladosporiumfulvum is dominated by one dominant gene.3.Resistance to race 1.2.3.4 of Cladosporiumfulvum was found to due to a single dominant gene through analyzing resistance behavior of the cross HN16(susceptible)×NHN42 (resistable).The results indicated that the diffence of two parents resistanc and susceptibility is obvious,the male parent HN42 shows resistance and the female parent HN16 shows susceptibility.The ratio of number of resistance and susceptibility on F2 is 3.17:1.00,which accord with the ratio of 3:1,which accord with the ratio of 1:1.The results shows resistance to Cladosporium fulvum is dominated by one dominant gene.4.Five AFLP markers and one SSR markers linked to the resistant gene Cf₁₉ were identified. The genetic distance of five AFLP markers on E38M61-2,E66M84-4,E66M44-1,E77M50-1 and 77M50-2 are 9.7 cM,10.7cM,12.75 cM,13.45 cMand14.4 cM differently.The genetic distance of one SSR markers on SSR318 is 12.5 cM.5.Four AFLP markers and one SSR markers linked to the resistant gene Cf₁₁ were identified. The genetic distance of four AFLP markers on E54M37-G,E62M1-A,E85M79-D and E62M59-G are 10.1 cM,12.3cM,14.5 cM and 18.8cM differently.The genetic distance of one SSR markers on SSR46 is 13.7 cM.6.Using AFLP marke to screen the tomato germplasms,at the same time identifying the resistance with race 1.2.3.4 in the greenhouse.There are 15 of 95 cultivars and lines were resistant tomato germplasm resoures.Among them 3 have Cf₁₉ gene,and 2 have Cf₁₁ gene.This the AFLP marker improved is credible.We should think that how to make use of them to breed resisting variety and to defend the loss of resistance.