Screening Of Germplasm Resource For Resistance To Multiple Disease And Storage Of Tomato

Tomato which include rich nutrition, good quality, wide application, high output, with a variety of beneficial to human body composition, have extensive planting area in the world. It is a kind of highly vulnerable to cause infection of vegetables, in recent years there were many wantonly popular diseases on tomato, the serious influence of yield and quality of tomato, normal processing for medicament control of plant disease, the prevention method is not complete, not long-term protection, also cause very serious pollution to the natural environment, at the same time, the tomato is easily damaged during the transportation and storage, which seriously affected their economic benefits. So, it is necessary for the market to breed multi-resistant and long shelf-life varieties.This experiment using marker-assisted selection and gene polymerization technology, we hybridize different tomato materials with different resistance genes and storage gene to aggregate multiple high quality genes. The we screen F4、F5 and F6 of 13485×13065 and 13485×13337 with SCAR 、 CAPS 、 Indel molecular marker technology in seeding stage. Combination seedling inoculation identification and agronomic traits in the field investigation in order to elect a variety of multi-resistant and long shelf-life tomato plant resources. What we done lay a solid foundation for breeding multi-resistant、high yield and quality tomato varieties. The results were as follows:(1) We use the female parent 13485(with Tm-1 、 Mi-1 gene), cross with male parent 13065(with Ty-2、Ty-3、I-2、Ph-2 and Ph-3 gene) and 13337(with rin gene) respectively,make the hybrid:13485×13065,13485×13337 and build six generation group.(2) We got 2-4 candidate markers that can separately identify Tm-1、Mi-1、Ty-2、Ty-3、I-2、Ph-2、Ph-3 and rin gene by review many literatures. Utilize male and female、hybridize generation and other materials to test the accuracy of markers. The final screening to determine the genetic distance is relatively small, at the same time also has a good stability and accuracy of molecular markers,the order is SCG12、M58、T0302、P6-25、Z1063、dTG422、TG328、R58。(3) In order to improve the efficiency of the molecular marker-assisted selection, we employed three co-dominant markers,P6-25,Z1063 and M-58,that tightly linked to tomato yellow leaf curl virus disease-resistant gene Ty-3,tomato Fusarium wilt resistant gene I-2 and tomato root knot nematode gene Mi-1,respectively,and we established a multi-PCR technique that can identify these genes simultaneously using the three markers.(4) Using selected markers to screen the F6 populations of 13485×13065 and 13485×13337. We identified the genotype and artificial inoculation of 836 plants from their F6 group by molecular marker assistant selection(MAS) technology in seeding stage. We got 27 plants with resistant to 5 diseases and containing seven resistance genes,There are 12 plants contain seven resistance genes were homozygous; We got 9 plants with resistant to 5 diseases and containing six resistance genes, including 1 plant not contain Ph-2,4 plants not contain Ty-3,4 plants not contain Ty-2 from the F6 population of 13485×13065. We also got 7 plants inbred strain resistant two diseases(including Tm-1、Mi-1 gene) and containing rin gene. We kept those single plants with good field performance and excellent fruit quality.(5) Select 200 materials of the 13485×13065 F6 generation to inoculate materials to inoculate Fusarium;And also,select tomato material 2 groups, each group of 100 plants of the 13485×13337 F6 generation to inoculate root-knot nematodes and tobacco mosaic virus. The results show that two kinds of detection methods of plant inosculation reached 86.5%, 88% and 88% in turn.