Comparatively Structure Studies Of Glycine Between Halobiotic And Common Environments

Using the technique of scanning electron microscope,light microscope and paraffin sectioned to study the structures of Glycine.L,which growing in different ecological environment of Baicheng suburb and Changchun suburb,i.e.halobiotic and common environments.The saline-alkali stress resistance experiment was carried out using G.lycine' s plants in the saline resistant and blackland.G.soja 50323—6;semi Semi-Wild soybean50338;Semi-cultur soybeanS0225& cultur soybean G.max.jNo.jiyu59。Through paraffin wax permanent slice' s two-dimensional optics transmission structure, scanning electron micro three dimensional spatial structure,segregation experiment and a series of experiments all prove that the structure evolve law of soybean belong to the salt adverse circumstance.The visualizations of actin in phloem sieve tube cells of Glycine plant root,including wild species G.soja Sieb.et.Zucc 50323-6,semi-wild species 50338,semi-cultivated species 50225 and cultivar G.max.Sieb.et.Zucc Jiyu 59 by scanning electron microscope was investigated.It suggests that actins broadly exist in phloem sieve tube cells of Glycine plant root.Under scanning electron microscope,actins present the regularity that changing from spherical body to cylinder then to filaments in experimental session.Based on immuno-gold labeling,treated samples with anti-actin in order to confirm the existence of actin in phloem sieve tube cells.The results show that the location and quantity of gold particles labeled actins were coincident with the actins observed by scanning electron microscope,however the control group which has not been labeled with anti-actin did not display any gold particles in phloem sieve tube cells of Glycine plant root,confirming the theory of Yan Longfei et al.that actins widely exist in eukaryotic cell.Few reports on views of distension and creeping of the stimulated actins under scanning electron microscope have been published so far.