Evaluation Of Physiological Function And Study On Ingredient Of Dark Tea

Dark teas are special tea in China.All Dark teas have post-fermentation processing and there are many microorganisms participated in the process.Therefore unique flavor and function to health had been produced.The study of Dark tea is still weakly and obstructed the further development of Dark tea.Therefore,Puer-tea,Liubao-tea,Fuzhuan-tea,Huazhuan-tea,Qingzhuan-tea,and Heizhuan-tea has been taken as study materials,and Tuo-tea,Mizhuan-tea has been taken as contrast materials,HTS has been used to evaluate their physiological function of them,HSCCC has been used to separate compounds of Dark Tea,HPTLC has been used to studies their liposoluble pigment and liposoluble fluorescence components.1.Two methods had been used to withdraw the composition material from tea.At the first method 95%ethanol was used to withdraw the composition material from tea and at second method 95℃water was used.Silica-gel has been used as holder for the ethanol liquor and solvent used to wish it,Petroleum ether Elution & Chloroform elution & Ethyl-acetate elution & Ethanol elution had been got.Aqueous extraction had been got by the second method.HPTLC analysis shows that Petroleum ether Elution compound composed with carotenoids and the Chloroform elution composed with pigment and alkaloids.Dark tea Aether-petrolei elution and the Chloroform elution can give fluorescence on 366nm light.HPLC analysis shows that the content of Gallic acid in Dark-balck tea is higher than in Tuo tea.And the content of Gallic acid in Pu-er tea is higher that in other Dark-black.Mi-zhuan tea also has high content of Gallic axid.And HPLC analysis also shows that Dark-black tea has high content of Theophylline,and the content of Theophylline in Hua-zhuan tea is 4 to 10 times higher than that in other Dark tea.2.To evaluate the faction of Dark tea,PPARδmodel,PPARγ,model,FXR activate model and FXR restrain model,LXR model had been taken on the study of Dark tea anti-activity on metabolizing Syndrome such as hyperlipoidemia, atherosclerosis,fatness & etc.And following conclusions had been got:(1) Fu-zhuan tea have been found being low pole.and have strong power on PPARd model,PPARγmodel,LXR activate model & LXR model.Therefore it promised potential therapy on hyperlipoidemia,atherosclerosis,fatness & diabetes mellitus would be found.Pu-er tea has good regulation of glyco-metabolism and lipid metabolism.(2) Liu-bao tea have good power on FXR restraint model,The mid-high components of Pu-er tea have good power on FXR restraint model,The mid-low components of Qing-zhuan have good power on FXR restraint model too.Therefore Liu-bao tea,Pu-er tea and Qing-zhuan teahas high value of being FXR inhibitor.And high pole of Dark tea have good power on FXR restraint model.(3) Tuo tea Ethyl-acetate elution can reinforce sensitivity of FXR restrain and it titles Tuo tea good foreground on medicine.(4) Tu0 tea Aqueous extraction has no power on PPARs model but Dark tea Aqueous extraction have.It is a hint that Dark tea can produce more water-soluble activity.components on anti-fatness,anti-hyperlipoidemia,anti-atherosclerosis than Tuo tea.And it hints that drinking Dark tea can prevent hyperlipoidemia, atherosclerosis and fatness.3 TNF,IL-1 model had been taken to evaluate the faction of Dark tea on anti-inflame,and the results show that Dark tea have no activity to TNF & IL-1.4 SGC-7901 cells,and HCT-8 cells had been taken to evaluate anticancer of Dark tea and the results show that all Dark-black tea have strong power on it.Dark tea Ethyl-acetate elution have strong restrain power on alimentary canal cancer.So it hints that eluting methods can be used on obtaining inhibitor of alimentary canal cancer from Dark tea.The inhibitor to HCT-8 cells in Dark-black tea has high pole than that to SGC-7901 cells.5.Jin-hua has a good physical activity on 5 NR models,The results of study show that the pole of Jin-hua activity components is broadly.The study on Jin-hua repressive power on alimentary canal cancer shows that Jin-hua has good antineoplastic effect.And the power in Chloroform elution is more stronger than in Ethyl-acetate elution.Furthermore there many function materials have been found in Jin-hua liposoluble Fluorescence Components.The study results show that Jin-hua is a precursor of medicine with anti-activity on metabolizing Syndrome and antineoplastic effect.6.A preparative high-speed counter-current chromatography(HSCCC) method for isolation and purification of Gallic acid,Theine,Theophylline from Ethyl acetate Elution was successfully established by using ethyl acetate-water as the two-phase solvent system.The lower phase was used as the stationary phase of HSCCC.The mobile phase used in HSCCC was the upper phase.With this solvent system,not only theine,Theophylline,Gallic acid be isolation,but also Catechin be separated.And with same solvent system,bidirectional elution mode has been used on separating Dark tea Aqueous extraction and the mode can short time of HSCCC analysis.7.In visible light,HPTLC analysis of Dark tea liposoluble pigment shows that 9 bands had been separated by aether petrolei-acetone(7/3,V/V) on Silica-gel plate. from high R_f to lower R_f lined asβ-Car(0.87),known pigment(0.74),Poa(0.65),Pya (0.53),Pob(0.49),Pyb/Cda(0.35),Neo(0.21).The bands of Dark tea have fluorescence on Rf=0.70.The light absorb character of Dark tea pigment are different from the light absorb character of pigment that said on article,and the ration or content in Dark tea are different from each other.8.According to pigment content,the cluster analysis of Dark tea by SPSS shows that the two green tea had been classed in one group,Liu-bao tea separated as a group and Dark-black tea and Mi-zhuan tea classed in one group.The result is satisfactory to the real.9.HPT1C analysis of Jin-hua liposoluble fluorescence components and found that the band on R_f=0.27 has three major light absorb on 241nm,288nm and 366nm, and the maximal is on 366nm.The band on R_f=0.58 has two major light absorb on 241nm and 190 nm,and the maximal is on 290nm.Using preparation TLC isolate liposoluble fluorescence and GS-MS analysis shows that the follows material in it: Docosane,Hentriacontane,Bis(2-ethylhexyl)phthalate,A-Neooleana-3(5),12-diene,Olean-12-ene,a-Neooleana-3,12-diene,4,4,6a,6b,8a,11,11,14b-Octamethyl-1,4,4a,5,6,6a,6b,7,8,8a,9,10,11,12,12a,14,14a,14b -octadeeahydro-2H-picen-3-one, 10,13-Dimethyl-17-(1,4,5-trimethyl-hex-2-enyl)-1,2,9,10,11,12,13,15,16,17-decahydr ocyclopenta[a]phenanthren-3-one,Squalene,Friedelin.