The Research On Capsicum Annuum L. Virus Diseases

The original type of pepper virus and the virus in different ecological zones of the hot pepper seeds production conducted virus around 11 provinces or municipalities of china.was detected and identified in this paper;the wide identification and screening of pepper germplasm resources was processed;and the seed treatment and pest control cultivation of pepper virus disease control,as well as the relationship between physiological indicators of pepper and virus disease resistance was studied,the results were as follows:1.The evaluation of original types of hot peppersTMV was still the main drug in the first original,ChiVMV rise to the second,CMV for the third,PVY for the fourth,ToMV for the fifth,BBWV-2 for the sixth,PMMoV for the seventh, BBWV-1 for the eighth,PVMV for the ninthThe phenomenon of complex infections was very common for Virus,only 12 samples were not infected with HIV between 237 samples,37 were infected with one virus,and the rest were infected two or more with the virus,the highest was infected with eight kinds of virus at the same time2.Detection of poisonous seeds.Two methods including RT-PCR and DAS-ELISA were adopted to examine CMV,TMV and ChiVMV virus of seeds.The Identification rate of CMV was 28.07%,The Identification rate of TMV was 26.31%,ChiVMV was not identificated;and the sensitivity of detection for DAS-ELISA was slightly lower than RT-PCR.The rate of virus-carring of thin cayenne pepper seeds was lower than pickle pepper and pointed pepper seeds;The rate of CMV and TMV infection were different in different maturing varieties,The rate of CMV infection was late varieties＞mid-maturing varieties＞early varieties,The rate of TMV infection was early varieties＞late varieties＞mid-maturing varieties.;The rate of virus-carrying of seeds in Hainan and Shanxi was slightly low.It was suitable for seed production.3.the identification and screening of pepper germplasm resources.By identifying the rust of seedling inoculation against CMV and TMV,for 203 copies of pepper,There were 1 highly resistant form to CMV,1 highly resistant form to TMV,26 resistant forms to CMV,32 resistant forms to TMV,11 resistant forms to CMV and TMV,27 resistant forms to one of the two,but moderately resistant to another.Meanwhile.After inoculation,the detection of relative virus content showed that the more serious were the symptoms,the higher was the relative content of virus,the lighter were the symptoms,the lower was the relative content of virus.4.The studies on correlation of physiological indexes and resistance of virus disease of the hot pepperThere were significant positive correlations between chlorophyll and cellulose contents and resistance to virus dieases in adulting stage;There were significant and positive correlations between protein contents and poeroxidase activity and resistance of to TMV in seedling stages,but were not significant correlations to CMV;There were not significant correlations between chlorophyll contents and Phenylalanine ammonia-lyase activity and resistance of virus disease in seedling stages.5.The Studies on the changes of some physiological indexes before and after inoculating CMV,TMV on resistant and susceptible varieties.The chlorophyll contents all elevated from the first day to the third day,and declined from the 5th day of post inoculation,the chlorophyll contents of resistant and tolerant varieties elevated from the 9th day post inoculation,While the chlorophyll contents of susceptible varieties decreased all the time.After inoculation,the changes of protein contents appeared to have decline trend after an initial ascent,but the period of ascent was different of inoculating CMV and inoculating TMV.The peroxidase activity increased slowly to the peak of enzyme activity then decreased,and the peak of enzyme activity was different between resistant and susceptible varieties.The phenylalamine ammonia-lysae had two enzyme activity peaks post inoculation,There existed a small peak from the first day to the second day,and the period of the second peak was the fifth day,and then it began to drop.The stronger was the resistance of varieties,the higher was the enzyme activity.6.The prevention to Capsicum annuum L.Virus Diseases with seed treatment and pest control cultivation.Different kinds of seed treatments had different control effects against virus diseases.The protection effect with two kinds of methods was better than the protection effect with one single method,and the effect of dry-hot,damp-heat and 10%Na3PO4 treatment in single treatment was better than hot water treatment,and the control effect and stimulation effect were obvious against virus dieases with pest control cultivation.