| Gynostemma pentaphyllum (Thunb.) Makino, also called Jiaogulan in China, is a perennial herbaceous vine that belongs to the Gynostemma genera in Cucurbitaceae family. As an important medicinal plant, Jiaogulan distributed widely in China and is a prolific resource. Gynosaponins are believed to be the main active components and play the crucial role for diverse pharmacological properties, such as nervous system diseases, cancer treatment and prevention. Gypenosides can be classified as etracyclic triterpenoid saponins, which haveβ-hydroxyl group at sites of C-3, C-12 and C-20. Glycosides normally located at site of C-3, C-6, C-20-bit, the rearrangement or cyclization of side-chain may easily arise and change into sub-glucoside after hydrolysis , for the internal acetal glucoside structure is vulnerable to acid, alkali and enzyme degradation.In order to discover novel compounds and study the active substance of sub-glucoside, this paper dealt with the ethyl acetate extracts after hydrolysis of gynosaponins. Silica gel column chromatography, reversed-phase chromatography and Sephadex LH-20 were employed for isolation and purification of hydrolysates. In this study , 38 compounds from Acid hydrolysates were afforded, 35 compounds were elucidated by means of spectroscopic techniques including 1D and 2D-NMR (1H-NMR, 13C-NMR, 1H-1H COSY, HMQC, HMBC and NOESY) as well as mass spectrometry (EI-MS, ESI-MS and HR-ESI-MS) in combination with the advanced database, including 10 new compounds, dammarane-type triterpen. They are:(3β, 23S) -3 -hydroxydammar-20, 24-dien-21-oic acid 21, 23-lactone(1-3),(3β, 23R)-3-hydroxydammar-20, 24-dien-21-oic acid-21, 23-lactone(1-4),(3β, 23R)-3 , 25-dihydroxydammar-20-en-21-oic acid 21, 23-lactone(1-5) , 3β, 20β-dihydroxydammar-22, 24-dien-21-oic acid 21, 23-lactone ( 1-6 ) ,3β,20β,25-trihydroxydammar -21-oic acid 21, 23-lactone(1-7) , gypegenin D(1-8) ,3β-hydroxy- dammar-24-en -23-carboxyl-21-oic acid (1-9),3β,20,23ξ,25-tetrahydroxy dammar-21-oic acid(1-10),3β,20, 21,25-tetrahydroxydammar -21-O-β- D-glucopyranosyl (1-11),3β,21ξ,25-trihydroxy -dammar-20-ene -21-O-β-D-glucopyranoside (1-12),(20S)-3β, 21ξ, 22ξ,24,25-pentahydroxy -21,24ξ- cyclodammarane (1-14). All the compounds were tested for their cytotoxicity assay of cell strain of MDA-MB-435, Hepg-2, HeLa with the method of MTT. The result showed that compound 1-3,1-4,1-8,1-32 have better inhibititory effect on MDA-MB-435 proliferation, compound 1-11,1-6,1-5 have better inhibititory effect on Hepg-2 proliferation, compound 1-6,1-7 have better inhibititory effect on HeLa proliferation. With Orlistat as a positive control, acid hydrolysates gypenosides were tested by inhibitory activity of the lipase, most of the compounds shows inhibit weak activity, just compounds 1-11,1-12 showed moderate inhibitory activity of lipase.Due to a wide variety of secondary metabolites from higher fungi and their biological activities, extensive attention has been paid to the researches of higher fungi both in China and abroad. However, there is no report on the chemical constituents and antioxidation activity of Cortinarius purpurascens. Cortinarius purpurascens, belongs to the family of Cortinariaceae and Cortinarius, is macrofungi fruiting bodies, distributed widely in Europe, North America, Japan and China's Heilongjiang, Jilin, Hunan, Sichuan and other places. This study tested anti-oxidant activity for different extracts of Cortinarius purpurascens, sampling at HeLanShan Natural Reserve by means of DPPH methods, result shows that ethyl acetate extracts have better activity. Pass through silica gel, Sephadex LH-20 and reversed-phase RP-18 column chromatography to afford 18 compounds from ethyl acetate extracts, 14 compounds were elucidated by means of spectroscopic techniques including mass spectrometry (EI-MS, and FAB-MS), as well as 1H-NMR. Including 9 of anthraquinone pigment, 1 Cerebroside, 4 ergot steroids compounds, they were first isolated the fungi. All the compounds were tested for their anti-oxidant assay with the method of DPPT, the result showed that compounds ufoolivacin (2-2), rufoolivacin D (2-3), rufoolivacin E (2-4), Leucorufoolivacin (2-5) has a strong DPPH free radical scavenging ability. |
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