Experimental Study And Clinical Study On The Treatment Of Hyperprolactinemia By Late Diagnosis And Treatment Of Postmenstrual Period. JOURNAL OF INTEGRATED TRADITIONAL AND WESTERN MEDICINE ON LIVER DISEASES

Title:Expenrimental and clinical studies from the perspective of PRL/PRLR-JAK2/STAT5 signaling pathway on treatment of menstruation caused by hyperprolactinemia using the method of soothing the liver and tonifying the kidneyObjective:This subject is to copy the model of rats with hyperprolactinemia, Self Chai Yu parasitism soup, Starting from the research angle of signal transduction, In the PRL/PRLR-JAK2/STAT5 signal transduction pathway as the core, The animal experimental study is to study the relationship between hyperprolactinemia factors and diminished ovarian reserve, explore the syndrome distribution rules of diminished ovarian reserve, and evaluate the efficacy of hyperprolactinemia by Traditional Chinese Medicine.Methods:â‘ Subcutaneous injection ofmaxolon was applied to establishing them ice model with hyperprolactinemia. Rats were at random divided into five groups, model control group, bromocriptine group, traditional Chinese medicine of high dose, middle dose and low dose, and one norm al group. Detect estradiol (E2) and follicle stimulating hormone (FSH) level in serum; and PRLR, STAT expression levels of ovarian were detected by western blot and Quantitative Real-time PCR,to explore the pathogenesis of hyperprolactinemia with psychosocial factors;â‘¡Analysis the frequency of Chief complaints and symptoms of hyperprolactinemia patients,to explore the syndrome distribution rules;â‘¢Compared the symptoms score before and after treatment,to observe the efficacy hyperprolactine by traditional Chinese medicine.Results:â‘ Serum E2 and FSH levels decreased in hyperprolactinemia model group, compared with the control group, with significant differences (P<0.05). While in the hyperprolactinemia model group, PRLR and STAT of hyperprolactinemia model group ovarian expressed decreased significantly (P<0.05).â‘¡compared with the hyperprolactinemia model group, Serum PRL levels decreased in every treatmeatal group, with significant differences (P<0.05); compared with the hyperprolactinemia model group,Serum E2 and FSH levels, PRLR and STAT of ovarian expressed increased significantly in ovary of treatmeatal group (P<0.05).â‘¢103 cases hyperprolactinemia patients were observed,the frequency of chief complaints: infertility(28.57%)>Delayedmenstruation(26.53%)>earlymenstruation(24.49%)>Hypomenorr hea (18.37%). the frequency of symptoms:Delayed menstruation (29.94%)> hypomenorrhea (28.74%)> infertility (26.35%)> early menstruation (9.58%)> amenorrhea (4.79%)> menorrhagia (0.60%). the frequency of symptoms:Kidney Deficiency and Liver Stagnation (47.75%)> Phlegm dampness stagnation (25.24%)> kidney deficiency and blood stasis (7.77%)> Qi stagnation and blood stasis (6.80%).â‘£To observe the clinical efficacy of soothing liver tonifying kidney treatment by"Chai Yu Jisheng Decoction" for hyperprolactinemia with late menstruation caused by liver depression and kidney deficiency, and to explore its mechanism.60 patients with hyperprolactinemia late menstruation were randomly divided into two groups, namely the observation group (Cai Yu Jisheng Decoction) and control group (bromocriptine) of 30 cases. Observe clinical efficacy of improvement of symptoms before and after treatment, evaluate and compare serum prolactin (PRL) and related hormones (E2, LH) levels. Results was that the levels of PRL of two groups are both significantly reduced and related hormones (E2, LH) have been benign regulated. But The clinical efficacy of the observation group in improving the level of hormone E2 and in improving Traditional Chinese Medicine Syndromes "liver depression and kidney deficit" is significantly better than that of the control group (P<0.05).Conclusion:â‘ yperprolactinemia can lead to reduced serum FSH and E2;â‘¡yperprolactinemia can lead to reduced the expression of PRLR and STAT in ovarian of female rats, Maybe it is one of the mechanisms of leads to diminished ovarian function;â‘¢delayed menstruation,hypomenorrhea, infertility, early menstruation are the the most common clinical manifestations of hyperprolactinemia patients; Kidney Deficiency and Liver Stagnation is a major clinical syndrome type of it;â‘£The markedly effective rate of hyperprolactinemia patients by "Chai Yu Jisheng Decoction"the total efficiency is 86.7%.and this treatment can also improve hormone level.so soothing liver tonifying kidney treatment by"Chai Yu Jisheng Decoction" is a safe and effective method of treating hyperprolactinemia.