Pathogenesis And Weak Laser Intervention Of Chronic Musculoskeletal Focal Point In Rats

Myofascial pain syndromes (MPS)is one of the most common types of musculoskeletal disorders. Its main symptoms are chronic muscle injury and pain, which not only affect the quality of patients’ life, but also cause great medical resource consumption, and has attracted more and more attention in the clinical treatment. Although the prevalence of the MPS is extremely high, its pathogenesis is still unclear, and the diagnostic criteria is lack of objective evidence. There is no uniform diagnostic criteria for the diagnosis of the MPS.In the laser therapy research of the MPS, the choice of treatment parameters often cause confusion and lack of specification. The common diagnostic criteria was described by Simons in 1990,including the palpation of the taut bands, the local twitch responses, and the patients pain recognition, active trigger point and spontaneous electromyographic activity. The pathological characteristics of the pain nodules or taut bands in the focal area of MPS is contraction knots,which is is mainly due to the excessive release of acetylcholine.The cotents of this study are including three parts.The first part is to explore the objectiveevidence. In this part, we use the Ultrasonographic elastography (SWE, shear wave elastrography) to detect the focal area of the MPS model rats, and then compare the effectiveness of the method with the electromyogram(EMG) method. The two methods are both effective in determining the focal area of the trigger points. This provides a new method for the diagnosis of the MPS. The second part is the exploration of the inflammatory mechanism in the rat model.Mainly through the verification of nuclear transcription factor KappaB/p65, COX2, voltage-gated calcium channels is high level expression in the trigger points of the rat model, and the nuclear transcription factor KappaB/p65, COX2 and voltage-gated calcium channels are also induced mutually, we can Preliminarily confirm the Simons’integrated Trigger Point Hypothesis.The third part is to explore the effectiveness of the Low Level Laser therapy in curing the MPS. In this part, we determine the sensitivity of the shear wave elastic modulus(SWEM) after we evaluate the effectiveness of the Low Level Laser Therapy (LLLT)in curing the MPS using EMG and SWEM. We also determine the wavelength plays a crucial role in the treatment process of the MPS using LLLT, through the results of the EMG, the SWEM and the Inflammatory factors such as KappaB/p65, COX2 and voltage-gated calcium channels.This provides a new evaluation method and a basis for the treatment of the clinical efficacy observation researches.At present, the study of the pathogenesis of the MPS is mainly based on the Simons’ integrated Trigger Point Hypothesis. In this study, we determine the effectiveness of the Integrated Trigger Point Hypothesis. We also determine the sensitivity of the shear wave elastic modulus(SWEM) in the MPS diagnosis. We order to provide a new method for the diagnosis of the MPS and a new understanding of the pathogenesis of the MPS.