The Evaluating Of Microvascular Geometry Of The Rat With The Double Staining Method

The Measuring of Microvascular Geometry of the Rat Heart Arteriolar and Vcnular Capillary Regions with the Double Staining Method Ph.D.Candidate Yang Shao-qing Advisor Prof.Liu Yi-li Abstract Objective: 1 .To provide a new histochemical technique for serveing to discriminate between arteriolar capillary (AC) and venular capillary (VC)regions on differences in enzyme activities in arteriolar and venular capillary portions ,the arteriolar and venular capillary portions express alkaline phosphatase (AP) and dipeptidyl peptidase IV (DPPIV), respectively. To provide new information regarding capillary network geometry in rat myocadium serve to understanding of oxygen transport to tissue and to evaluate the effect of some pathyological stress on the geometry of capillary networks.Sometimes the capillarity of left ventricular tissue of rats subjected to coronary artery occlusion and the old rat by the way of the double staining method.Up to date,relative report was not discovered in the nation. .2.To serve to gain a three-dimensional architecture of the myocardial microvascular casts were prepared by perfusion of the coronary arteries wtih methyl methacylate. The species variation of the coronary arteries among the human, pig and rat was evaluated. Methods: 1 .The double staining technique was established in the normal rats.Sixteen male Sprague-Dawley rats were used in this study (body mass300?0g) After anesthesia with sodium pentobarbital(4mgIl OOg i.p.),the colored microspheres with a diameter of l5jim,were injected into the coronary artery of the hearts,and then hearts were rapidly excised, wighcd and dipped in optimum cutting temperature compound(O.C.T.compound.) solution,frozen in liquid nitrogen and prepared for cryostat sectioning. ? ? INIOrpfloffiCtflC iatVSCS OI CSDlllllfltV lQ iVOCVTCS WCfC i8QC ill hC SCCtloflslIOI tllC ICflVClltflCIC ffiVOCatQllllll．,．lflC SCCtlollS CfltCfCQ tllC DYOgflllTI OI QOllblCIStsllllflZ iQ ffiffillllofllstOCflCffilC81 lflVCSllZatloll OI tflC卜MJ 8．－－Q乙AIJ4．ffillflflIOI VSSClllat CflQOtllCllllffi CCll．L．foe IICflft WSS CXDOSCQ t．－－xvllQfl ICIt－SldCdltflof8COtoffiV lfl SOQll．－wx－－ DClltODatDlth卜iCStflCtlZCQ lQ VCfltllatCQ fats．tflC ICltlICOfofl3fV ［if［Cry WSS OCCIWCQ tVltti 8 ilfl SllttllC lQ tllC TfltjryHIVSSAllOSCQ IOflltflC CXOCflthCllt8I SllDICCtS,q3QnyS srtCf tlC SlllRCfy hC flCflf［ WSS fCffiOVC lQICfOSS－SCCtloflS WCfC i3QC IOf ITIOrpflothCITIC i3lVSCS．tlC Sll8111－ODCfatloll fatsl 工七e丸瞪fRHt,Werycolltrola抡内把饨．3qm旧气用琐臼dhea戊s models olthehtnnan．maldrid fat WCfC DfC口W yy－口CTlllSIOfi OI iC COfofl8YV flf［CflCS Rqth inC'utoMlllmetnacrvlate resoectlvelv．Ine aata of moronometry of coronary arlenai treeslgnQ ttriCC－QlthCflslollai tC口CtCCtllfC OI ffilCfOVSSClllfltllfC DV SCflll．－－lllR CICCryollllttilCfOSCODV OI ffilCfOCOfTOSIOll GSStS WCfC COllCCtCQ．lD ＊e吕＊！雹吕:且．川0e ＊0r口区别ftz ＊a口1llflfV 口＊D口mnsfC＊SI乙＊八s)WC fC DD sknihc用寸Iv reduced In VCre旦ions(p＜0．01P)．＊S corn口red 在o AC DlfCZIOllSv乙 YCZIOllS WCfC Cfl8IflCtCrtZCQ lflCTCdsCQ CPlllflfV QCflslTVID Jn＜0．01＼shoner seIZTTlem lenoh,a二了eaer diameter(口＜o,o》,cppHgr sunace DD densl何Ic＜0．0且):an口 a Increase InCapH8fV sur二ace oenslr卜 且ne 辽on:lloslw．DD hdex wassi囚dhc田V比仁刃eenA...