The Studies On Mitochondrial Ultrastructures And MtDNA～(4977) In Prostate Tissues From BPH Patients

Benign prostate hyperplasia (BPH) occurs in male elders commonly and frequently, which does harm to lift quality of male elders with its complications. The incidence rate increases year by year. Up to date, little is known about the exact etiology of BPH. Aging and functional testis are the two element factors epidemiologically. Much has done for the mechanism of testis in BPH, however, little does of aging.Aging is a complex phenomenon characterized by energy deficits. It was hypothesized that these deficits might be due to defects of the mitochondrial oxidative phosphorylation system (OXPHS), and mtDNA mutations are responsible for it. The 4977bp deletion(mtDNA4977, or common deletion) was the first and commonest mutation among these mutations. As we know, there is no study on mitochondrial ultrastructures or mtDNA mutation with human prostate tissue.With this study, we are trying to set up relationship between mitochondria and BPH, and investigate how aging plays a role in BPH.Part oneThe study on mitochondrial ultrastructures inhuman prostate tissues with BPH Objectives: to investigate the changes of mitochondrial ultrastructuresin human prostate tissues. Materials and methods: Experimental group includes 10 samples,which comes from the prostate tissues by prostatectomy.The Control group includes 5 samples, all young (<50 y),from the death by accident. Fresh tissues were done withimmobilization and stain, then observed in TEM.Mitochondrial damage score was evaluated at the sametime..Results: 1. we found mitochondrial ultrastructures changes a lot in the experimental group, which includes swelling of mitochondria, incontinent membrane, and ridge becoming flat or diminished; damage scores are much higher than the control group. (P<0.05).2. in experimental group mitochondria damage score is related to Qmax. (P0.05).3. mitochondria damage score is not related to age(P>0.05).Conclusions: 1. mitochondria ultrastructures injuried in prostate tissues of BPH patients.2. the changes of mitochondria may be related with urinary obstruction from BPH.Part twomtDNA4977 in human prostate tissues Objectives: 1. to investigate mtDNA4977 in prostate tissues with BPH.2. to investigate if there is age-dependented mtDNA4977 inhuman prostate. Materials and methods: Experimental group includes 48 samples,which comes from the prostate tissues by prostatectomy; while the contrast group 24 samples, all young (<50 y), from the death by accident. Total DNA of prostate was extracted, and PCR was made with the sample DNA amount 1ug. In experimental group when mtDNA4977 exists deletion rate (mtDNA4977/total mtDNA) was measured by serial dilutions PCR.Results: 1. incidence rate of mtDNA4977 is much higher in BPH group (P0.01).2. incidence of mtDNA4977 is related to age, size of prostate and Qmax of patients.3. incidence of mtDNA4977 in human prostate is positive related to age.Conclusions: 1. incidence of mtDNA4977 in BPH is much higher than the control, which is associated with patient's age, size of prostate and Qmax.2. there is age-dependented mtDNA4977 in human prostate tissue.