Induction Of Anti-leukemic Cytotoxicity By Dendritic Cells Derived From Cord Blood In Vitro

Objective: To induce cytotoxic T lymphocytes(CTLs) by dendnttc cells derived from cord blood m vitro Methods: Cord blood mononuclear cells(CBMCs) were cultured in vitro with the combination of various cytokines Dendrittc cells were confirmed by their morphology, immune phenotype and capacity of stimulating the MLR(mtxed lymphocyte reaction) CTLs were generated through the coculture of T lymphocytes and dendrttic cellsassays were performed for the measurement of cytotoxicity of CTLs Results: The distribution of the subgroups of I lymphocytes m cord blood is similar to that in adult peripheral blood The quantity of dendntic cells in CBMCs is very low, merely 0 41% During culture dendritic cells evolved in morphology, and become larger, more irregular in shape Spiny dendrites or multiple cell processes emerged on the surface of dendntic cells There were 28 4% dendntic cells among the cultured cell populations at d15 Dendritic cells derived from CBMCs are capable of stimulating the proliferation of allogeneic lymphocytes in MLR CTLs derived from autologous T lymphocytes, which were induced by dendritic cells pulsed with lysates of HL-60 cells, possess specific cytolytic effects against HL-60 cells Conclusions: Relatively high percentage of dendntic cells can be generated under our culture system Dendntic cells derived from cord blood are able to induce the production of anti-leukemic CTLs in vitro...