Adenovirus Mediated Angiostatin Gene Therapy For Rat Glioma

Objective To study the therapy for C6 glioma with the recombinant adenovirus containing angiostatin eDNA (AdhCMV- AGS). Methods Angiostatin gene was cloned into vector pAdCMV-AGHpA to make pAdhCMV-AGS. Recombinant adenovirus was constructed in 293 cells contransfected with pAdCMV(AGS) and pJM17. Detected the effect of AdhCMV-AGS on proliferation of endothelial cells(ECV3O4) in vitro. Made a model of rat C6 brain glioma, then observed tumors size and survival of tumor-bearing rats treated with AdhCMV-AGS. Results AdhCMV-AGS could inhibited the proliferation of ECV3O4 cells significantly, but not C6 cells. The size of tumors injected with AdhCMV-AGS decreased. Some of it even disappeared, while the size of control (injected with saline solution) was larger significantly. the survival time of groups were beyond 90 days (with AdhCMV-AGS), 16.8 ±3.3 days(with Ad-null), 16.7±2.5 days(with saline) (p<0.001). Conclusion AdhCMV-AGS could inhibit the proliferation of endothelial cells and the angiogenesis by which it suppressed C6 glioma. Ours results suggest that antiangiogenesis gene therapy will be a promising way of treating tumors that have abundant capillary.