Galactose-PEI-Iron Oxide Nanoparticles-mediated Angiostatin Gene Therapy For Hepatocellular Carcinoma

With the development of molecular biology, Antiangiogenosis therapy for tumor is especially regarded in recent years, Gene therapy of angiostatin on tumor has become a "heat spof"of study at present. Hepatocarcinoma is of abundant blood supplement due to its double vessel system. Therefor, if it is treated by antiangiogenosis therapy, wonderful curative effect will be acquired. Significance of antiangiogenosis therapy for hepatocarcinoma lies in getting rid of plight of conventional treatment method and forming a new strategy for hepatocarcinoma.However, gene therapy need to be accomplished by ideal vector, virus vector and non-virus liposome vector are of limitation at present, they are not competent for in vivo transfection gene therapy, it is important that an ideal gene vector is studied.Recently, the coming of nanoparticles as gene delivery carriers provides possibility for overcoming above-mentioned difficult. Comparing with conventional gene vector, nanoparticle is of particular advantage. It is not only of some obvious advantages such as excellent biocompatibility, stronger capacity of carrying drug, high safety, easily preparation, low cost and so forth, the transfection efficiency and targeting capacity could be also enhanced by surface modification of the nanoparticles. Therefor, more and more evidences show that nanoparticle is a promising gene delivery vectorIn this study, we prepared galactose-PEI-iron oxide nanoparticles vector with double targeting capacity utilizing Fe₃O₄ as core, PEI as carapace and galactose as modifier. The results indicated that the nanoparticles were of characteristic of sphere shape and a mean diameter of 46nm. In the range of 200μg/ml, it was safety to tissue/cell. While the weight ratio of nanoparticles with DNA was 3:1, the encapsulating efficiency of DNA was about 95% or so. While PH value was 7, combining capacity of nanoparticles with DNA was of the highest stabilization. Galactose-PEI-iron oxide nanoparticles were of some characteristics such as magnetic compliance, biocompatibility. long circulating function and so on. Comparing with...