Degenerate Studies On The Partial Cartilage Damage Of The Longitudinal Crack And Horizontal Defect On The Weight-bearing Area And Non Weight-bearing Area Of The Medial Femoral Condyle On Rabbit

Objective: This research text studied the pathology changes of the partial cartilage damage of the longitudinal crack and horizontal defect on the weight-bearing area and non weight-bearing area of the medial femoral condyle on rabbit; in order to be clear and definite the difference of the different harm form on the weight-bearing area and non weight-bearing area of the articular cartilage. Materials and methods: Making the animal model of the longitudinal crack and horizontal defect on the weight-bearing area and non weight-bearing area of the medial femoral condyle on rabbit. On the medial femoral condyle of rabbit, the foreside of the articular cartilage were non weight-bearing area, the rearward of the articular cartilage were weight-bearing area. Of 42 rabbit, respectively making the longitudinal crack and horizontal defect on the weight-bearing area and non weight-bearing area of both meidal femoral condyle . Seven animals were killed at each of the following intervals: 0 week, 1 week, 4 weeks, 8 weeks, 16 weeks, 20 weeks after the operation. Sections were stained with hematoxylin and eosin, safranin 0 and alcian blue, to compare the difference of the different harm form on the weight-bearing area and non weight-bearing area of the articular cartilage.Results:1.Macroscopic Observation:The superficial defect in articular cartilage could be readily located in all joints collected at various intervals. There was no evidence of an obvious reparative process in any animal. From 0 to 20 weeks, the horizontal defects of articular cartilage appeared slightly smaller than the original size and the depth became shallow; in places the junction between these two zones couldstill be discerned but often this line of demarcation was difficult to determine and the margin of the defects was no longer sharply demarcated from the surrounding cartilage. From 0 to 20 weeks, the margin of the longitudinal crack longitudinal crack was no longer sharpness but appeared circle bluntness and the depth of defect became shallow.2.Hematoxylin and eosin stain showed: The longitudinal crack of non weight-bearing area on the medial femoral condyle remained in the Separate condition after the operation. From 0 to 20 weeks, the cell nucleus stain around the incision varied by different degree from navy blue to shallow blue to gray; the cell nucleus became by different degree from pyknosis to fragmentation to obliteration; the chondrocyte form, structure and number of the base and the nucleus stain were in the normal condition. The incision of the longitudinal crack of weight-bearing area on the medial femoral condyle were stop upped by pink material and bonded together, looking like healing. Other lesion was similar to the longitudinal crack of non weight-bearing area on the medial femoral condyle. After the operation of the horizontal defect of weight-bearing area on the medial femoral condyle , from 0 to 20 weeks, the cell nucleus stain in the surface layer of the defect varied by different degree from navy blue to shallow blue to gray; the chondrocyte lacuna, cell and cell nucleus became by different degree from small to atrophy to obliteration. The cell lacuna, cell and cell nucleus in the germinal layer on the base became by different degree from small to atrophy to obliteration and the cell number decrease. At one week, four weeks and eight weeks, the deep stain around some cell lacuna increased around the defect and the cell lacuna enlarged with compensation. The lesion of the horizontal defect of non weight-bearing area on the medial femoral condyle was similar to that of weight-bearing area.3. Safranin-0 staining showed: After the operation of the longitudinal crack of non weight-bearing area on themedial femoral condyle, from 0 to 20 weeks, cartilage matrix red around the incision obliterated by different degree, the cell number of male decreased by different degre and the red around the chondrocyte lacuna decreased by different degree; the matrix staining in the germinal layer became superficial...