The Effect Of NO And Ps In Ischemia/reperfusion Induced Pulmonary Injury

Part 1 Dynamic Changes of NO, NOS and Ps afterpulmonary ischemia and reperfusionAIM: To suty the dynamic changes of No, NOS and Ps after pulmonary I/R and to observethe lung injury induced by I/R. Method:1. Rats were divided into four group randomly Group A: Control groupGroup B: Sham group, suture was only placed in left hilusGroup C: Ischemia and reperfusion group (I/R group), according to reperfusion time after 60min ischemia,.it is divided into five sub-group: RO.SI RI ?Ra?R6> Ri2 stand for 30min, In, 3h, 6h, 12h after reperfusion.Group D: righ lung group after left lung I/R, five sub-group as group C.2. We use the model of warm ischemia and reperfusion in situ in single lung.3. The content of NO in vascular wall and plasma were measured according to themethod of Ignarro and shechter, immunohistochemisty of eNOS, iNOS, Ps and ELISA forplasma Ps.Result:1. There is no difference between left and right lung in the content of NO, eNOS, iNOS, Ps in vascular endothelium and Wet/Dry ratio in lung tissue.2. After pulmonary I/R, the content of NO in vascular wall and plasma decrease first and then increase and reach a peak value at 6h of post-reperfusion and begin to decrease at 12h of post-reperfusion.3. eNOS after I/R decrease first and then increase to reach a peak value at Ih of post-reperfusion. iNOS increase at Ih of post-reperfusion and reach a peak value at 6h of post-reperfusion.4. Expression Ps in vascular endothelium rapidly become strong and PPs increase after Ih of post-reperfusion.5. The content of NO and eNOS in right vascular wall after left pulmonary I/R increase slightly and iNOS hasn't change.6. Wet/Dry weigh ratio increase after 3h of post-reperfusion and reach a peak value at 6h of post-reperfusion and begin to decrease at 12h of post-reperfusion.7. Blood oxygen pressure begin to decrease at 3h and reach a lowest value at 6h and begin to increase at 12h of post-reperfusion.8. Pulmonary H-E stain show edma and neutrophil infiltration in alveolar tissue, there isn't change in group D.Conclusion:1. The content of NO in vascular wall and plasma decrease first (<30min) and then increase and begin to decrease again at 12h of post-reperfusion.2. eNOS expression decrease first and then increase to reach a peak value at Ih of post-reperfusion, but iNOS expression increase only after Ih of reperfusion and continue for a long time.3. Ps expression in vascular endothelium rapidly increase within 30min of post-reperrusion and reach a peak value at 6h of post-reperfusion.4. Wet/Dry ration increase, blood oxygen pressure decrease and edema formation with neutrophil infiltration in H-E stain indicate injury of lung tissue after ischemia and reperfusion.5. The counter-side lung can been effected slightly but it don't induce structural and functional change after lung I/R.Part 2 The effect of L-arg, L-NAME on pulmonaryischemia and reperfusion injuryAIM: To study the effect of L-arg, L-NAME on ischemia and reperfuion induced pulmonaryinjry Method:1. Rats were divided into four group randomlyGroup A: low-dose L-arg treated group (L-arg I group) lOOmg/kg L-arg were gived iv before I/R and it was divided into five sub-group: RO 5 > Rp RS. Re?Ri2-Group B: high-dose L-arg treated group (L-arg II group) lOOmg/kg iv first and then lOmg/kg min L-arg iv continuous, also it was divided into five sub-group: Ro5, Rp R3, Re. R12.Group C: Low-dose L-NAME treated group (L-NAME I group), lOmM L-NAME was administrated iv before I/R, it was divided into five sub-group: RO.S. Rp R^, Re> RU-Group D: high-dose L-NAME treated group (L-NAME II group), lOOmM L-NAME was administrated iv before I/R, it was divided into five sub-group :Ro.5> RI> RB?Re> RH-2. The method to measure content of NO in vascular wall and plasma and Ps in vascular endothelium and plasma as described above.Result:1. The content of No in vascular wall and plasma increase in early stage...