| Biocompatibility is the most important factor for biomaterials to use in treatment. Up to now, it is not always to see the design and study for the new type biomaterials, and truly basing on the molecule design. So the biomaterials which are used in treatment, particularly the materials which use for actifical machines are in the situation being used only, faring away from application. At the beginning of 1990s, several study groups include Matsuda et.al firstly pointed out the surface modification of medical polymer, materials-photo-chemical immobilization, which was dramatically different from any other surface modification process available for medical materials. They used this method to improve the surface lubricity, antithrombogenicity, antibacterial, nettability and cell growth activity of the biomaterials, get a series of photo-chemical immobilized biomaterials which had good biocompatibility.In this paper, we also used this new method to modify the surface of cell culture polystyrene. By the incorporation of two active molecules, two new photo-immobilized biomaterials were synthesized. We studied the application possibility of these two kinds of materials in cell culture engineering and biomedical engineering areas.In section one of the present thesis, it was the first time to synthesis the thermoresponsive polymer AzPhPIA which had photo-activity and the low critical solution temperature was only 21.5. The results showed that, in serum-free cell culture system, the AzPhPIA-Pst film was suitable to be applied to detach cells instead of trypsin. But in serum cell culture system, AzPhPIA-PSt surface could not detach cells completely. Two typical serum proteins were used to find the reason. The results showed that it was fibronectin which increased cell attachment so that cells could not be detached, and albumin didn't influent the cell detachment, but influented the adsorption of fibronectin, Surface analysis demonstrated that the coated protein would not affact the wettability of AzPhPIA . The function of AzPhPIA could not resist the function of fibronectin so that the application ofthermoresponsine biomaterial in serum cell culture system is limited .In section two, it was also first time to systhesis phycocyanin(PC) anti-tumor polymer . We extracted PC from Spirulina platensis . By photo-chemical immobilization , phycocyanin was immobilized on polystyrene surface , the inhibition activity to liver cancer cells 7402 was enhanced significantly . When the immobilized amount of PC was 0.02 mg/well , the inhibition activity of immobilized PC was 55% , compared with 6% of PC solution . Selenium had cooperativity for immobilized PC in certain concentrations . The co-immobilization of other proteins inhibited the cancer inhibition activity of PC, enhanced liver cancer cells growth. Photo-chemical immobilization of PC also inhibited pancreatic cancer SW1990 cells growth effectively with 40% inhibition rate. Normal method was used to purify the polysaccharide of Spirulina platensis, PSPI(MW23000) was got. PSPI had cooperativity for immobilized PC to inhibit pancreatic cancer cells. When the concentration of PSPI was 17.5 mg/L , the inhibition rate could be 50% , compared with 40% of PC group. More than that, in this paper we firstly used Cu2+ dative adsorption and ion exchange methods to purify polysaccharide of Entrromorpha Clathrata Grev . Polysaccharides with 98.4% quality and 97.8% recovery ratio were obtained . The method was more simple than other method with good purification effect and low cost. One of the five kinds of polysaccharides, could inhibit SW1990 cell growth effectively. IR spectrum showed that the polysaccharides(in) which were extracted with hot water was acidity polysaccharides. This polysaccharide(MW34500) had good cooperativity for immobilized PC. The highest inhibit rate was 80%, compared with 40% of immobilized PC.
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