| Section 1. A Comparative Study of Necrosis and Apoptosis of Hepatocellular Carcinoma Cells after TACE with Low-dose and Conventional-dose Chemotherapeutic AgentsObjective To study necrotic rate and apoptosis index of hepatocellular carcinoma (HCC) cells after transcatheter arterial chemoembolization (TACE) with low-dose and conventional-dose Chemotherapeutic agents. Methods Thirty patients with resectable HCC underwent superselective TACE. Twelve cases (Group A) received low-dose Chemotherapeutic agents:2 - 4mg mitomycin C (MMC) was used in the condition of the tumors diameter < 5 cm,MMC 4 - 6 mg,epirubicin (EPI) 10 mg in 5 - 8 cm,and MMC 6 - 8 mg,EPI 10 mg,CBP 100 mg in > 8 cm. Conventional-dose of Chemotherapeutic agents (MMC lOmg,EPI 40mg and CBP 300mg) was given in Group B (n=18). Lipiodol- Chemotherapeutic agents emulsion was injected into the feeding arteries of tumors followed by gelatin sponge (GS) or polyvinyl alcohol (PVA) particles embolization. The tumor resection was subsequently performed 5 - 30 days after TACE. The necrosis rate and apoptosis index of tumor cells were evaluated by pathologic examination and TdT-mediated dUTP nick end labeling (TUNEL). Group C (n=20) was treated by tumor resection without TACE. Results Necrosis rates in three groups were 90.5 + 9.0%(A),87.9 + 9.5%(B) and 5.0 + 4.5%(C) respectively. There was no significant difference between Groups A and B(t=0.788,.P>0.05),the significant difference was found between Group A and C (35.749,P<0.001),Group B and C (t=36.458,P<0.001). Apoptosis index in the three groups were 10.25 + 5.65%(A),11.33 +4.17%(B) and 4.29 + 3.02%(C) respectively. There was significant difference between Group A and C (t=4.094,P<0.001),and Group B versus C (t=5.504,P<0.001). No significant difference was observed between Group A and B (t=0.360,P>0.05). Conclusions Superselective TACE for HCC with conventional-dose chemotherapeutic agents does not significantly enhanced the anticancer effects compared with use of low-dose in necrotic rate and apoptosis index observation.Section 2 Evaluation of the Effects on Survival Period of TACE with Low-dose versus Conventional-dose Chemotherapeutic Agents in Hepatocellular CarcinomaPurpose:To evaluate the effects on survival of TACE with low-dose versus conventional-dose chemotherapeutic agents in HCC patients,and to analyzed effecting prognostic factors. Materials and Methods Eighty-two patients with unresectable HCC underwent superselective TACE. Patients in Group A (n=40) received low-dose chemotherapeutic agents. Patients in Group B (n=42) were given conventional-dose of anticancer agents. Tumor response and survival period in two groups were compared. Cox proportion-hazard modeling was used to evaluate the relative importance of prognostic variables. Results There was no significant difference between the two groups in initial tumor response (P<0.05). The median survival period in all 82 patients was 18 months (mo),in Group A and B were 20 mo and 16 mo respectively (P>0.05). The cumulative survival rates at 6,12,18,24,30 mo were 68.4%,57.6%,38.4%,26.6%,19.9% in Group A,and 62.6%,43.8%,31.9%,26.5%,26.5% in Group B. There was no significant difference in survival rate in the two groups (P>0.05). The factors influencing prognosis were Child-Pughscores (P<0.0001),tumor emboli in the portal vein (P<0.0001),tumor size (P <0.0001),modalities of embolization (P<0.0001),TACE times (P<0.001). The dosage of anticancer agents in TACE was not relevant to the survival rates (P=0.883). Conclusions There was no significant difference in tumor response and 6 - 30 mo survival rates in superselective TACE with low-dose and conventional-dose chemotherapeutic agents for treating HCC .Section 3 The Effects of the Different Dosage of Chemotherapeutic Agents on T Cell Subsets Post-TACE for Treating Hepatocellular CarcinomaPurpose To investigate the effects of the different dosage of anticancer agents on the changes of hosts'T cell subsets after TACE in HCC. Materials and Methods TACE was performed in 36 patient...
|
PDF Full Text Request