Effects Of Shen-Kang Injection On Cultured Rat Kidney Interstitial Fibroblast

Objective: To observe the effect of Shen-Kang injection (SKI), a preparation of traditional Chinese medicinal herb on kidney interstitial fibrobiast (KFB), and to approach the action and mechanism of preventing renal interstitial fibrosis. Method: Using Lotensin as positive control and KFB of rats as study object, we observe the effect of different SKI concentration on KFB proliferation and fibronectin secretion , TGF-β1, collogen as well the adjustment of its cell cycle and apoptosis by test methods such as MTT colorimetry, ELISA method, 3H-TdR, flow cytometry and DNA agar gel electrophoresis. Results: (1) SKI has obvious dose-dependent inhibit effect on rats KFB proliferation, FN auto-secretion and collogen synthesis(p<0.05, p<0.01), and the effect is better than Lotensin especially when high dosage; (2) SKI can dose-dependently inhibit rats KFB secreting TGF-b1(p<0.05, p<0.01) remarkably, the effect is better than Lotensin when high dosage; (3) SKI can increase the G0/G1 ratio gradually, decrease the cell ratio in S period, and prevent cells access to S period from G1 period as well promote their apoptosis, and the effects of high-dose group and moderate-dose group are better than Lotensin. Conclusion: SKI can prevent kidney interstitial fibrosis in vitro. The mechanism probably relate to directly acting on KFB, inhibiting its proliferation and TOF-fr secretion, as well as decreasing the deposition of FN and collogen, and preventing cells access to S period from G1 period and inducing their apoptosis.