In Situ Expression Of AP-2 And MnSOD In NSCLC And The Regulation Of MnSOD Expressions By AP-2 In Lung Cancer Cells

Chapter I: In situ expression and significance of AP-2 transcription factor and the human manganese superoxide dismutase (MnSOD) in non-small cell lung cancerObjectives: To study the expression of AP-2 transcription factor and MnSOD in non-small cell lung cancer (NSCLC) and their relationship with tumor biological characteristics.Methods: 50 NSCLC tissue and 15 normal lung tissue were used to detect the expression of AP-2 transcription factor and MnSOD with an immunohistochemical method.Results:(l)The positive rate of AP-2 was zero in normal lung tissue but 32% in NSCLC tissue. It indicated that the expression of AP-2 in NSCLC tissue was erevated (P<0.05).(2)The postive rate of AP-2 was significantly higher in NSCLC patients of pathological grade 111(57.89%) than in those of grade I -11(16.13%) (P<0.05), and in patients of clinical stage III-IV (44.83%) than in those of stage I -11(14.29%) (P<0.01), and in NSCLC patients with lymph node metastasis(40.54%) than in those without lymph node metastasis (7.7%) (P<0.05).(3)The positive rate of MnSOD was 70% in NSCLC tissue but 100% in normal lung tissue. The expression of MnSOD in NSCLC tissue was lower than that in normal tissue(p<0.05). The expression of MnSOD was declined as tumor progressed. The positive rate in T3.4 (47.83%) was lower than in T1-2(88.89%) (PO.05).Conclusion:(1)The up-regulation of AP-2 expression in NSCLC tissue suggests that AP-2 may be involved in NSCLC carcinogenesis.(2)The AP-2 expression was associated with tissue pathological grade, clinical stage and lymph node matastasis in NSCLC. It demonstrates that AP-2 may play an important role in the occurence and progression of NSCLC.(3)The down-regulation of MnSOD expression in NSCLC tissue suggests that MnSOD may play an important role in the pathogenesis of NSCLC. Perhaps it is a tumor-suppression factor in NSCLC.