| Windstroke is a coromon and difficult disease. The TCM treatment based on the differentiation of syndromes owns the superiority in the therapeutic effect. The following is the study on the pathogenesis of windstroke ?Heat-toxic Affecting Brain Collaterals and the mechanism of Qingkailing in treatment of windstroke ?Removing Heat-toxic from Brain Collaterals.1. PurposeTo study the pathogenesis of windstroke ?heat-toxic affecting the brain collaterals. To observe the formula compositions ?baicalin, gardenoside, and chlorogenic acid and their targets ?neurons and neuroglia cells, to research the effect of the compositions of Qingkailing on the survival rate of neurons and neuroglia cells damaged by ischemia, to discuss the interrelations between the compositions and the two important target neurons and neuroglia cells of the central nervous system, with the application of microcosmic and macroscopic analysis and by way of the combination of reduction and entirety and the organic separation of the complicated formulas and organism. In addition, to study the biological fundamentals of the mechanism of Qingkailing in removing heat-toxic from the brain collaterals for the treatment of windstroke from the viewpoints of traditional nervous protective agents and the regulation of endogenous activated substances (NGF and TNF-a) in order to reveal the reasonability of compatibility of formulas and to clarify the superiority and features of Chinese medicine and pharmacology.2. MethodsTheoretical method: The literature and logical analysis are adopted in the research of "heat-toxic" and "collateral" in the duration of windstroke.Experimental method: The focal cerebral ischemia of the rat is produced by ligation. HPLC is applied to determine the volume of the effective compositions of Qingkailing permeated the blood-brain barrier (BBB) under the pathological conditioa The pharmacodynamic experiment on cells is done with the permeated compositions by means of MTT, ft-TDR incorporation, radioimmunoassay, ELISA, and immuno-fluorescence to observe the effect of the total formula and each composition of the formula on the cortex and the survival rate and reproduction rate of the hippocampus neurons and the neuroglia cells damaged by ischemia, the effect on the neuroglia cells secreting theendogenous active substance (NGF and TNF-a), and the action pattern of the medicine on cells. 3. Results3.1. The theory study: According to Prof. Wang Yongyan, the dysfunction of zangfu is the fundamental and the disordered qi and blood are the inducing factors of windstroke, in which the heat-toxic affects the collaterals of brain. He puts forward that the basic principle of treatment for windstroke is to remove the heat-toxic from the collaterals on the basis of modern biology. 3. 2. The experimental study:3.2.1. Study on the compositions of Qingkailing permeated the BBB under the condition of ischemic damage.In the normal and pseudo-operation groups there is a small amount of baicalin, gardenoside and chlorogenic acid permeated the BBR The quantity of baicalin, gardenoside, and chlorogenic acid permeated the BBB in the ischemic group is remarkably more than that of the normal and pseudo-operation groups (P<0.01). It means that the effective compositions enter the central nerve system, reach the damaged area, and act on the neurons and the neuroglia cells. The substantial pharmacodynamic bases of Qingkailing are baicalin, gardenoside, and chlorogenic acid.3.2.2. The effect of the compositions of Qingkailing on the survival rate of hippocampus and cortex neurons damaged by ischemia.Three days after ischemia, the survival rate of hippocampus and cortex neurons is decreased (P<0. 05). The survival rate of hippocampus neurons in the total formula and baicalin, gardenoside and chlorogenic acid groups is higher than that of the ischemic model group. There is an obvious difference among the groups(P<0. 05). The survival rate of cortex neurons in the total formula and baicalin and gardenoside groups is higher... |
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