The Regulating Characteristics Of Progesterone And 18-methyl Levonorgestrel On Osteoblastic Cells

Part One Expression of progesterone receptors in human osteosarcoma cell line MG63 and human normal osteoblastsObjective: To isolate and identify the functions of the human normal osteoblastswhich were obtained from the trabecular bone of normal subjects, and study theexpression of progesterone receptors in human osteosarcoma cell line MG63 andhuman osteoblasts.Methods: The human osteoblasts were isolated from the trabecular bone bycollagenase digestion. The expression of alkaline phosphatase, collagen type I andmineralized nodes were studied with modified Gomori's assay, Van Gieson's stainingand alizarin red-S staining, respectively. The expression of PRA and PRB mRNA andprotein was measured by semi-quantitive reverse transcriptase polymerase chainreaction (RT-PCR) and Western blot in human osteosarcoma cell line MG63 andhuman osteoblasts.Results: The isolated human normal osteoblasts had the characters and functions ofosteoblasts in vivo. PRA and PRB mRNA and protein were expressed in both humanosteosarcoma cell line MG63 and human osteoblasts.Conclusion: The cultured human osteoblasts hold the osteoblast phenotype, andhuman osteosarcoma cell line MG63 can be used as a cell model of osteoblast. MG63cells and human osteoblasts may be influenced by progestins.