The Influence Of Different Concentrations Of Glucose And Insulin On The Human Osteosarcoma Cell Line Mg63 Activity

Osteoporosis (OP) and diabetes mellitus (DM) are the most common diseases of endocrinology. Osteoporosis (OP) is a metabolic bone disease characterized by low bone mass and deterioration to microarchitecture of bone, leading to an increase of bone fragility and fracture risk. Diabetes mellitus (DM) is featured by hyperglycemia with deficiency of insulin or resistance to insulin. As the complex mechanism of OP and DM, different manifestations of osteopathy are observed in DM. In type 1 DM, the bone mass is decreased compared with the healthy subjects. In type 2 DM, the bone mineral density (BMD) measured by dual-energy X-ray absorptiometry (DXA) may be decreased, normal or elevated.In this research, we take an insight into the influence of glucose and insulin to human osteosarcoma cell line MG63, hoping to give an explanation to the mechanism of OP in DM.We set 5.5mmol/L 10mmol/L 20mmol/L 40mmol/L 4 levels of glucose and 0mol/L, 10^-7 mol/L 10^-6 mol/L 10^-5 mol/L4 levels of insulin, and then combined them into 16 culture media of different glucose and insulin concentration. Human osteosarcoma cell line MG63 was cultured in these media successively for 21 days. The proliferation rate, secretion of ALP and BGP, percentage of apoptosis cells and the formation of calcified nods were observed.