| Gastric cancer has been the most common malignant tumor in our country.How to inhance the five year survival after the radical operation has been the key point of their treatment.Modern molecular biology shows that the multi-change of the related gene and the retardation of cell apoptosis have played the leading role in canceration course. Meanwhile, cancer research shows that the aim of chemotherapy is how to induce the cancer cells to apoptosis, by wipping out or helping to kill the residual malignant cells after an operation or radiotherapy, so as to decrease the remanent cancer cells.Due to the unknown genesis and the causes of gastric cancer, reasons of this malignant disease have still remained unclear, so what we can diagnosis and offer treatment to mostly suffers in an advanced stage.Moreover, the multi-drag-resistant and the characteristic of highly malignancy often lead the remenant lesion to relapse recurrence and to metastasis, which always result in prognosis mala. Therefore, how to enhance the cure rate and to prolong survival has been a challenge to basic medical researchers and clinicians since. In the experiment, we designed and carried outour research plan by combining rhGH with DDP and /or MM-C to induce the MFC to apoptosis. During the experiment, we made use of the techniques of FCM TEM, molecular biology, and DNA gel electrophoresis to detect and check the situation of apoptosis, which had been induced under the above methods.In the first part of our experiment in vitro,by means of MTT and IC50, the tumor killing power was evaluated and measured as shown below:In the group of rhGH combined with DDP and group of rhGH with MM-C the inhibitory rate was 86.65% and 69.33% respectively, while to the group of single DDP and the group of single MM-C the inhibitory rate was 46.70% and 42.68% respectively. IC50 of rhGH with DDP and rhGH with MM-C were 100 g/ml, 125 g/ml respectively. It is obvious that the killing power of rhGH combined with DDP on the gastric cancer cell is much stronger than the usage of single DDP, (p<0.01).By means of FCM, the peak of apoptosis isclearly shown while the ladder-like apoptosis chart has also been clearly shown in DNA gel electrophoresis. Moreover, films of ECMhave shown the typically morphologic changes related to apoptosis on the samples.In the second part of our experiment in vivo, the established transplantable model of MFC put into the 615 rats had been made, and then, the ready drugs were applied to thedesigned groups respectively.In this way ,we studied and analyzed the drug action on the process of apoptosis induction in vivo so as to draw up a way to treat cancer sufferers around the time of peri-operations. Methods: MFC was inoculated under the fore armpit of 615 rats (N = 120) until the transplanting tumor was grow up and stable.We treated the rats with rhGH with DDP or /MM-C by making use of single DDP and single MM-C respectively. After a given treatment cause, the tumor was inhibited, and the life prolonged rate, data of apoptosis and survival condition and drug effects had been through a comprehensive evaluation.Results: Group of rhGH with DDP, rhGH with MM-C have shown higher cancer treating effect. The tumor restricted rate was 78.70% and 48.10% respectively.Tumor in the test group had been apparently inhibited (p<0.01). The survival span of tumor bearing rats were apparently prolonged (p<0.01). The experiment has shown that it is possible to induce apoptosis in gastric cancer in a suitable way, and that the method would be especially helpful in the cancer suffers' treatment, when they are treated with rhGH combined with certain anticarcinogens. |
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