DNA Chip Analysis Of Gene Expressing Spectrum In Human Urinary Bladder Transitional Cell Carcinoma

Bladder neoplasms are the most common tumors of genitourinary tract in China and among which the transitional cell carcinoma is the most frequently encountered pathological type with the distinctively high recurrence rate. The rapid developments in the field of immunology, molecular genetics, biochemistry and molecular biology have greatly deepened our expertise concerning the process of tumor origination and progression at the cytological and molecular level. Recent years many scientists have witnessed a large quantities of researches on the relationship between activaton of oncogenes, loss of cancer suppressor genes and microsatellite DNA unstability etc and the process of tumor origination and progression in the field of oncology in order to explore the cytological and molecular mechanism of tumor origination and progression. Despite a lot of valuable experimental evidence in hand,it has been commonly manifestated that it is incorrect to interpretate many steps in the process of tumor origination and progression with only single molecular or gene change and the currently widely accepted notion is that the process of tumor origination and progression involves complicated, multisteps and multiple genes. The gene chip, a brand-new and ever sophiscated technique developed in 1990s provides a powerful engine to large-scalely and highly-throughput research on the gene changes in the process of tumor origination and progression and marks the emergence of genome biology era.With the aid of gene chip.people can conveniently observe the expressing variations of tens of thousands of target genes or gene segments in just but oneexperiment.In order to explore the correlation between the variations of gene expressing spectrum and the process of tumor origination and progression ,this experiment takes advantage of gene expressing spectrum chip and takes normal bladder mucous membrane tissue as control to find out the variations of gene expressing spectrum among normal bladder mucous membrane tissues, TCC tissues and recurrent TCC tissues, and then confirms partial identical genes expressing in the contrary direction within the primary and recurrent TCC tissues via semi-quantity RT-PCR.The main results and conclusions are as follows:1. Controlled by normal bladder mucous membrane tissues, there are obvious up regulation in 229 genes' expression and obvious down regulation in 170 genes' expression of gene expressing spectrum of primary TCC tissues. The differentially expressing genes involve a variety of categories including oncogenes and cancer suppressor genes, cytological signal transduction genes, cyclin genes, cytological regulating protein genes, apoptosis related genes, DNA synthesis, repairing and recombination protein genes, DNA binding, transcription factors genes, cytological superficial antigens and adhesive protein genes, ion channels and transportation protein genes, and metabolism related genes.2. Controlled by normal bladder mucous membrane tissues, there are obvious up regulation in 175 genes' expression and obvious down regulation in 168 genes' expression of gene expressing spectrum of recurrent TCC tissues. The differentially expressing genes involve a variety of categories including oncogenes and cancer suppressor genes, cytological signal and transduction genes, cyclin genes, cytological regulating protein genes, apoptosis related genes, DNA synthesis, repairing and recombination protein genes,DNA binding,transcription factors genes, cytological superficial antigens and adhesive protein genes, ion channels and transportation protein genes, andmetabolism related genes.3: There are 165 jointly differentially expressing genes in both primary and recurrent TCC tissues, among which there are joint up-regulation genes and joint down-regulation genes. The differentially expressing genes involve a variety of categories including oncogenes and cancer suppressor genes, cytological signal transduction genes, cyclin genes, cytological regulating protein genes, apo...