The Research Of The Respiratory Function Reconstruction In The Rats With Upper Cervical Spinal Cord Injury

Objectives:1 . To lay the microanatomical foundations for respiratory function reconstruction by transposition of accessory nerve to reconstruct the function of phrenic nerve in the rats with upper cervical cord injuries.2. To study the accessory nerve, the phrenic nerve and their branches histochemically and count the motor fibers in the rat.3. To study the features of the motion potentials of the diaphragm in the normal rats, thus the optimal site can be detected in the diaphragm .4. To establish the experimental model of the respiratory function reconstruction in the rats with upper cervical cord injuries through transposition of accessory nerve and phrenic nerve reconstruction. The function testing method of the diaphragm is adopted to measure the motion function of the diaphragm postoperatively.5. To observe the histochemical change of the diaphragm after the respiratory function reconstruction in the rats with upper cervical cord injuries through transposition of accessory nerve and phrenic nerve reconstruction.6. To observe the histochemical change of the phrenic nerve after the respiratory function reconstruction in the rats with upper cervical cord injuries through transposition of accessory nerve and phrenic nerve reconstruction.7. To study the features of the motion potentials of the diaphragm after therespiratory function reconstruction in the rats with upper cervical cord injuries through transposition of accessory nerve and phrenic nerve reconstruction.8. To study the features of the blood gas changes after the respiratory function reconstruction in the rats with upper cervical cord injuries through transposition of accessory nerve and phrenic nerve reconstruction. Ultimate Objectives:To investigate the feasibility of respiratory function reconstruction through accessory nerve transposition and phrenic nerve function reconstruction in the rats with upper cervical cord injuries, so new method can be adopted to reconstruct the respiratory function in the patients with upper cervical cord injuries in the clinical practice. Methods1. The bilateral accessory nerves, phrenic nerve, and their branches of 30 male rats were dissected under operating microscopy with 16-time-magnification. The diameter, length of the accessory nerve and its branches, the distance between the accessory nerve and the main trunk of the phrenic nerve were measured with the slide gauge with precision of 0.001 millimeter.2. The microscopically anatomized accessory nerves, phrenic nerves and their branches, 60 in total, were AchE histochemically stained with Kamorsky-roos method. The motor fibers were counted with VIDS-III graphical analyzer.3. One EEG electrode was placed subcutaneously in the midline of the skull as stimulate electrode and the other EEG electrode was inserted into hard palatesubmucously as cathode in thirty male rats respectively. And one square wave electrical pulse was stimulated with myoelectrical graphical machine. The stimulating intensity was 15 mA, the wave length was 0.2 ms, and the stimulating interval was 200 ms. Along the inferior border of the rib, centralized as xiphoid, two incisions with 1 centimeter long were made. And the abdominal side of the diaphragm was totally exposed. With naked eyes, the eccentric circular electrode were pierced into the following eight sites: the medial, intermediate, and lateral crus, the junction of the posterior axilla line and the inferior border of the eleventh rib, the junction of the anterior axilla line and the inferior border of the ninth rib, the junction of the intermediate line of the clavicle and the inferior border of the eighth rib, the junction of the para-sternal line and the inferior border of the seventh rib, the sterni. The MEP of each point was recorded respectively. The optimal sites of the diaphragm MEP were found and recorded.4. 180 male adult-rats were divided into 6 time-based groups (form the first month to sixth month with one month interval) at random. They were subsequently divided in...