A Study Of The Inhibition Effect Of Ultrasound And Its Mechanism On The Proliferation Of Human Ovarian Cell COC1

Objective: To investigate the Effect of Pulsed Focused Ultrasound (PFU) on Cell Lysis of Human Ovarian Cell Line COC1 and the Relationship between its effect and Free radical formation attributable toultrasound .Methods: Human ovarian cell line COCl were exposed to PFU[ a frequency of 0.8MHz, with an intensity level of 83.6W/cm2 Pulse Average intensity, Pulse duration(PD) 20us-100us]during various exposure times and immediately tested for cell viability by MTT colormetric assay. The effect of free radical scavenger on cell viability was explored. Free radical formation due to Ultrasound exposure was investigated by High Performance Liquid Chromatograph(HPLC) combined with electrochemical detection.Results: COCl cells viability decreased with the prolongation of sonication. In same duration of sonication, COC1 cells viability exposed to PFU (PD100us,75us and 50us) significantly decreased compared with that exposed to PFU (PD25us). COC1 cells viability exposed to PFU (PD 50us) did not significantly changed in the group where free radical scavenger were administrated as compared with control group. Aqueous solution containing salicylic acid was irradiated by PFU (PD50us) with different duration. The amount of 2,5-DHBA assayed by HPLC combined with electrochemical detection increased with the prolongation of sonication. The amount of 2,5-DHBA exposed to PFU (PD 100us and 75us) significantly increased as compared with that exposed to PFU (PD 25us) in same duration of sonication.Conclusion: PFU killed human ovarian cell line COC1 through celllysis. COC1 cells viability exposed to PFU decreased with the time prolongation and the increased pulse duration of sonication. Free radical attributed to ultrasound cavitation did not play the role.