| Preparations of nanoparticles as controlled drug delivery system have been widely studied. Nanoparticles are those microparticles that their diameter between 1-1000nm and carry drugs. Because of good liver-targeted, nanoparticles will be good carrier of anti-hepatitis virus. Chronic hepatitis is common disease all over the world, espassially hepatitis B. We have prepared JMS effective extraction by capital 248 fund. Althouth better anti-virus activity in vivo, part plasma drug concentration is low and effection is poor. Developing a liver-targeted nanoparticle system can increase treatment effection.On the base of a vast amount pharmacology, we selected anti-virus effective fraction. Meanwhile, main compound named JMG was separated and identified, which was new compound by international message reference.Extraction technic was selected by orthogonal design. Technic condition : extraction three times by five times 70 percent alcohol, one hour every time; D101 resin refined, JMG's saturation ratio was 2.28mg/g absorbtion ratio was 2.27mg/g. Washing by water, end point was determined by Molish reaction. Washing by 30 percent alcohol, end point is determined by TLC. Washed by 70 percent alcohol, JMG's eluation ratio was 1.98mg/g. In the end, alcohol wais recycled, extraction fraction was dried. JMG was determined by HPLC. At the same time, seven residues from D101 resin were determined by headspace GC. Results indicated that D101 resin was safe.We prepared stable and uniform liver targeted Potentilla Anserine Lextraction polybutylcyanoacrylate (JMS-PBCA-NP). To optimize preparation conditions by orthogonal design, and prepare JMS-PBCA-NP by emulsion polymerization method. JMS-PBCA-NP was uniform and round, and the average diameter range was 100-200nm, the embedding ratio was 81.97%. The preparation process was stable and feasible.JMS-PBCA-NP according to the optimum preparative conditions were prepared. The results of observing throuth TEM showed that nanoparticles were almost spherical and the diameter was between 100nm and 200nm. The Z average mean diameter, intensity mean diameter, volumn mean diameter, number mean diameter, polydispersity, zeta potential, were 106.8nm, 228.5nm,227.0 nm ,190.9 nm ,0.29,-20.5mV, they were determined by photon correlation spectroscopy(PCS). The loading efficiency and encapsulating efficiency were 51.23%,81.97%. According to studies above, it was concluded that the most drug was carried by nanoparticles.In order to nanoparticles system's stability and liver-targeting, JMS-PBCA-NP freeze-dried injection was prepared. According to studies above, nanoparticles' shape, diameter, loading efficiency and encapsulating efficiency were stabile, and vitro release curve presented obvious sustained release character.To study the distribution of JMS-PBCA-NP in visceras of mice. The concent of JMS-PBCA-NP was found to be 78.22% in liver of 15min after iv admistration. Pharmakenetics results indicated that drug distributed quickly, t1/2 , was 0.19h,and eliminated very slow, t1/2 was 198h.Uptake of hepotocytes was used to study the permeation of JMS-PBCA-NP cross the liver cell membrane. The cell uptake by rat hepotocytes in vitro demonstrated that nanoparticles could greatly increase the amount of JMSuptaking by cell. Pharmacological experiment showed that JMS-PBCA-NP injection could obviously promote the anti-hepotitis virus effection of vitro 2215 cell and duck model that was caused by toxin, and had better effect compared with the JMS injection.In order to achieve hepatic targeting of JMS nanoparticles galactosyated human serum albumin were prepared by covalent coupling lactose to'HAS using reductive amination method, as legend of ASGRP. The number of galactose residues was determined phenol-sulfuric colorimetric method and MADLI-TOF-MS method. When the number of galactose residues is small, the results of two metbods were consistent. When the number is big, the results of phenol-sulfuric colorimetric method should be adjusted.The work is going.
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