| The quality of human beings is absorbed more and more attention with the development of economy and improving of living condition. While in our clinical work, we can often experienced such kind of newborns with fetal growth restriction (FGR) due to the factors of either mother, fetus or placenta. Some of them will suffer from hypoxia ischemia encephalopathy (HIE) , even long-term sequences of neurological system. Whether this kind of FGR babies has their brains damaged during the restriction intrauterine life, the degree of this damage and whether can we perform some interference methods during intrauterine developing periods? No definitive answer can be found nowadays both at home and abroad. So they are our objectives in these papers. Our whole study was divided into four parts: Part I deals with the rat model of FGR; Part II relates to the expression of both growth associated protein-43 ( GAP-43 ) and S-100pin FGR pups' brain , the two proteins play important roles in the fetal cerebral development , damage and repairmen!. The long-term capability of study and memory were investigated in FGR rats by water maze test and long term potentiation (LTP) of hippocampus was reported in Part M. While in part IV, we discussed the protective effects of basic fibroblast growth factor (bFGF) given on the brain of FGR rats.Materials and methodsWistar rats were provided by China Medical University Animal Center. Pregnancy rats were divided into 2 groups, unilateral maternal uterine and ovarian arteries were clamped at gestational day 13 in group 1, while in group2, the clamping was performed in day 17, pups in the left undamped side served as control. The clamping persisted 30mins,then reperfusion, and the pups of bothstudy and control groups were sent back to the abdomen that was then being sutured . At day 21, cesarean section was performed and the pups were taken out, weighted and the development was estimated. In part II ,the cerebral expression of GAP-43 and S-100mRNA was studied by the method of RT-PCR. In part HI, Morris water maze test was used to detected the long-term capability of study and memory in FGR rats; by the changes of LTP at the hippocampus, we can i-dentify the damage of nervous system and the definitive effects of FGR on the capability of study and memory. We detected the protective effects of bFGF given to the FGR pups in part IV, in this part, we performed bilateral clamping at ges-tational day 17, group 1 was clamped for SOmins, group 2 served as sham control group, group 3 was operated the same as that of group 1, while during the operation, at the first and second day after operation, bFGF was given intrab-dominally(4u/g) respectively. All the pups were taken out by cesarean section at day 21. After birth weight and cerebral weight being examined, the expression of GAP-43 and S-100pmRNA were detected.ResultsPart I ; Whether the clamping performed at day 13 or 17, the body weight of pups in the study group was obviously restricted. The body and cerebral weight of pups in group 1 was 3. 095 0.777 gand 0.150 0.024g, decreased 19. 56% and 8.29% respectively than those of control group(3. 803 0. 663g, 0. 175 0.019g). The decreasing is significant, P <0.05,P <0.01. The body and cerebral weight of pups in group2 was 3. 391 0. 485g and 0. 152 0.029g, decreased 11. 83% and 14. 12% respectively than those of control group(3. 846 0.526g,0.177 0.024g). the decreasing is obvious, P <0.01.Part II : The expression of GAP43 and S-100pmRNA: GAP-43mRNA in FGR pups was 1.002 0.151, which was significantly lower than that of control (1.189 0.117 ) , P < 0.01. while the expression of S-100 mRNA just showed the opposite trend, which was significantly higher in FGR group (1. 204 0.145) than that of control(0.967 0.179), P<0.01.Part in: In the Morris water maze test, both of the two group rats showedsimilar patent phase, no obvious difference could be found. While from the second afternoon of training, the capability of study and memory in FGR pups decreased obviously, the patent per... |
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