Discussion On Experimental Animal Model Of HSP And Its Clinical Symptomatic Grading & Quantifying Standards

Objective:In order to make the study on Henoch-schonlein purpura(HSP) of TCM standardization,establish the experimental animal model and discuss its clinical symptomatic grading and quantifying standards. Methods:i)Establishing the animal model of HSP :Gliadin being taken as food antigen,meanwhile reticuloendothelial system being blocked by India ink,these two methods were applied to make mouse under the condition of clearance deficiency in reticuloendthelial system and mucosal immunological system continously stimulated ,so HSP animal model was finished.ii) Experimental study on HSP prevented and treated with huaban granule:Different dosages huaban granule was applied to prevent and treat HSP,indexes such as microscopic hematuria,quantitative examination of urinary protein,RES function,CIC and immunopathology,etc.were tested,the control group was given Cimetidine.iii)Discussion on the symptomatic grading and quantifying standards of HSP:Applying the method of experts' evaluation,screening the main clinical indexes according to instructive principle of clinical study on traditional and new Chinese medicine,defining their weight and making estimation about their reliability based on the contribution of main and minor symptoms,then judging patient's condition and classifying into three grades :light,middle and severe,making the symptomatic grading and quantifying standards.Results:i)HSP model was established successfully.ii)The pharmacodynamic characteristic of HSP treated with huabangranule might be the regulation on the immunological efffect,thekey to prevent and treat injury of kidney induced by HSP wascombing prevention and treatment,prevention was the first,Chineseherbs and Westernal medicine were combined to apply in theclinical treatment of HSP.iii) The symptomatic grading and quantifying standards of HSPbelonged to heat of excess type was set up,the patient's conditionwas classified clearly.Conclusion:i)IGA nephrosis model caused by gliadin can be taken as the modelof HSP.ii)Huaban granule can effectively prevent HSP and the regulationon immunological effect maybe one of the machanism.iii)The making of symptomatic grading and quantifying standardsprovides the basis for the standardization of clinical study on HSP.