Study On Antilipemic And Anticoagulant Preventing Necrosis And Apoptosis Of Osteocytes In Steroid-induced Osteonecrosis Of The Femoral Head

The stetoid induced necrosis of the femoral head takes the first place that non traumatic necrosis of the femoral head, account for 2/3, and have the tendency to continue increasing.For a long time, people are studying the pathogenesis of the stetoid induced necrosis of the femoral head , propose theories is for instance osteoporosis, high pressure, lipodystrophy disorderly, blood vessel congeal, cytotoxic of osteocytes ,apoptosis of osteocytes etc, But its exact mechanism has not been very much clear yet , have been influenced preventing and treating the stetoid induced necrosis of the femoral head. At present, most scholars think that the hormone causes the lipodystrophy to cause the osteonecrosis of the femoral head , Some scholars think that it is one of the importants cause of disease too that it is disorderly to congeal the system of dissolving. In recent years foreign scholar reportses in femoral head necrosis bone find amount of apoptosis of osteocytes, further to explain the pathogenesis of the stetoid induced necrosis of the femoral head.In recent years, a lot of scholars have designed many kinds of method to reconstructed blood transport of femoral head, expect to make the pathological change delay , static even take a turn for the worse , thus keep the femoral head, But there is not a kind of effective prevention method. Scholars begin to study antilipemic preventing steroid- induced osteonecrosis of the femoral head, Have received better result. The purpose of this text lies in probing into the method of a kind of more effective prevention the stetoid induced necrosis of the femoral head and mechanism.A. set up the necrosis of the femoral head model of the mouse , and watch from the respects, such as histology, ultra micro-structurea. mouse divide into groups, steroid- induced osteonecrosis of the femoral head foundation and treatmant.Divide the animal into four groups: Ⅰ: given the dexamethasone , lumbrokinase and lovastatin ( n =20)Ⅱ: given the dexamethasone and lovastatin ( n =20)Ⅲ: given the dexamethasone ( n =15)Ⅳ: blank contrast( n =5)A group given the dexamethasone (2mg), lovastatin (0.014mg) and lumbrokinase (6ku) Once a week. B group given the dexamethasone (2mg) and lovastatin (0.014mg) Once a week. C group given the dexamethasone (2mg) Once a week. Behind the eight week, it tract to death and come out hip joint laterale incision, Appear the head of femur, cut off the thigh bone of underpart of head of femur, take out the head of femur, split having the form along precededding , watch the gross attitude of head of femur. Fixed in the neutral formalin liquid . At half the head of femur samples, routine dehydrate, soak wax, wrap up and bury, cut into slices conduct dye by HE, Mere mirrors watch head of femur change and choose 3 a piece of high power fields of visions wantonly draw half take a photograph of blocks of field of vision ask and publish empty bones fall nest proportion and make film by morphology. At the remaining half head of femur sample, calcium are taken off by EDTA, two fifth regular aldehyde dehydrating , epoxy resin make burying, a pair of dyeing, The electric mirror of transmission watches the change of the bone cell of head of femur.b. resultThe histology watches on the whole: The section of head of femur of A, B and D group is red, does not have obvious difference, the section of head of femur of C group is pale red.Histopathology watch: bone trabecular of head of femur of A and B Relatively C thick, abundant red blood corpuscles ; The empty bone lacuna of C group increases obviously than A, B two groups, A group is: 3.25±1.1％, B group is: 7.32±1.87％, the two relatively have remarkable differences ( P< 0.001). C group is: 23.51±2.42％, A group is 1.51±0.95％, very closer the D group.Ultrastructure watch: In the A and B group, osteocytes is abundant, nucles is relatively normal, chromatins is homogeneous,organelles is abundant and there are many and clear collagenous fibrils; In the C group, osteocytes is relative...