| Objective:The curative effect of Xing Nao Qi Zhi capsule (it is called XNQZ for short. It is contributed by chuangxiong, shichang pu, ju luo and gouqi) was proved, and furthermore its function mechanism was probed into through serum pharmacological method. Method:Part1. Praxiology and pathomorphology in mice model introduced by repeated ischemia reperfusion injuryThe healthy ♂Kunming mice were divided into two groups, the false surgical operation group and the model group, by random. The model was duplicated by blocking the blood and the Repeated Ischemia-Reperfusion twice. While the false surgical group is only a process. The blood wasn't blocked and the tail wasn't bleeded. After testing the praxiology on the 7 days, 15 days, 30 days after the operation, irrigate and fixed the heart with 4% paraformaldehyde. The tissue from optic chiasm to nipple was taken, covered with paraffin, maken into coronary slice, dyed in HE and nissli and observed under the optical microscope.Part2. Effects of Xingnao Qizhi Capsule on praxiology and pathomorph- ology in repeated ischemia reperfusion injury miceThe healthy ♂Kunming mice were divided into 6 groups, the false surgical operation group, the model group, the high dose group of XNQZ, the low dose group of XNQZ, the gingko leaf group and the Nimodipine group. On the second day after the operation, 0.5% carboxymethy cellulose Antrim or the corresponding medicine was given by wateredstomach. After testing the praxiology on the 7 days, the 15 days and the both day after the operation, the material was taken to observe its pathological morphology. The method was the same as that in the first part. Part3. Protective function of XNQZ Drug-containing serum on the cell PC12 injury.The healthy ♂big rats were selected by examination and divided into 5 groups by random, compared serum group and drug-containing groups (4 groups with different dosages). The drug-containing was made by giving medicine 3 days continuously by watered stomach twice a day, with an interval of 12 hours.After the cell PC12 recovered and full grew on the bottom of the culture bottle and the culture liquid was abandoned. It was washed with liquid of D-Hand's and digested by liquid of trypsin. Then the digest liquid was abandoned. The culture liquid of 1640 was used to stop digesting. Blow and beat to make the cells asunder and count under the discrepancy microscope. Then the culture liquid of 1640 was used to dilute the liquid to 5×105/ml. The cell was inoculated in 24 bores culture plate. After the cells grow full of the single layer, the original culture liquid was abandoned and the cells was washed with the liquid of D-Hank's and balanced a certain time b putting in the liquid of 1640 with 5% different dosage of XNQZ in serum. Then different damage liquid was put damage liquid anoxic damage liquid ox radical damage liquid, Caf liquid GLU damage liquid, and NO damage of the growth and form of the cells PC12 was observed under the upside down discrepancy microscope, the activity of LDH on the pure liquid was measured by contrasting-color method and the vitality of the cells was measured by MTT method.And each form of the damage repress rates were calculated.The above experiment established the model group (blank drug- containing) and compared group at the same time (blank drug-containing, but didn't add the damage term, and other terms were the same as the experiment groups).Part4. Effect of XNQZ-containing serum on PC12 cell apoptosis and apoptosis gene induced by hypoxia1. Measured by TUNELIt was the same as before to make drug-containing, culture the cells PC12 and induce the anoxic damage. The sticking wall cells collected were washed with the liquid of PBS and drawn on the carrier glass plate treated by poly-lysine in advanced and blew to dry, fixed with the solution of 4% paraformaldehyde incubated at room temperature with penetrating liquid incubated in the ice, TUNEL reacted mixture incubated in wet box owners- POD incubated in wet box, showed the color by D...
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