A New Method To Find Unknown Chemokine Ligand And Its Clone & Expression

Background & Aim: Chemokines have well characterized proinflammatoryactions, the involvement of chemokines in inflammation are well established, but theirfunctional role in disease progression, and particularly in the development of liverfibrosis, are not yet well understood. In this experiment we investigated the functionalrole that CCR5 plays in inflammation and the progress to fibrosis during hepaticfibrosis. Methods: We developed and revised a murine model of immune complexinduced hepatic fibrosis for this syndrome. Using this model, we examined themorphology, immunohistochemistry (CCR5) and the serum ALT/HA level differencebetween the model and anti-mCCR5-Abs treated group in the progress of liverfibrosis. Results: The data showed that significantly increased expression of CCR5were observed within hepatic fibrosis immunohistochemistry sections, after thedisease initiation, especially at the end stage of hepatic cirrhosis. Blocking thefunction of CCR5 with an anti-mCCR5-Ab resulted in slower progression of liverfibrosis, which was confirmed by morphology and serum ALT/HA level examination. Conclusions: These results highlight a novel role for CCR5 in formation anddevelopment of liver fibrosis, and indicate that in addition to recruiting inflammatorycells this chemokines may critically involve in irreversible tissue damage.