The Experimental Study Of Bone Marrow Stromal Cells (BMSCs) Co-cultured With Neural Stem Cells(NSCs) And Intracranial Transplantation In Rats

The incidence rate of brain injury is increasing year by year following the development of traffic. This disease is paid close attention more and more on health and life of human. But at present the existent treatment measure is not ideal to many patients. So any enhancing damaged neural function after brain injury is the focal point of study now. Recent years because the study of stem cells is performed some result we find new hope. The study of neural stem cells and bone marrow stromal cells is one of important researches in neurology . Many studies hope to understand the regularity of growth and regenerated on embryo and adult by the correlated study of MSCs and NSCs, make the disease corrected with cell transplantation and gene transduction cured by the intrinsic character of cells.The indeed of the concept of neural stem cells confirm fully the imagine of growth and regenerated on nervous system in the past 50 years. The finding of adult NSCs hint brain tissues existing repairing function by itself. After brain injury endogenetic neural stem cells are activated to help recover the neural function, and ectogenetic stem cells' transplantation repair the damaged neural function.But the source limit makes ectogenetic NSCs not be applied widespread. BMSCs can secrete many factors and stimulate the differentiation of endogenetic NSCs, and there are more than 30 reference articles about MSCs itself differentiation into neuron. BMSCs can be gained from himself bone marrow, avoid exogenous material and obstacles of law and ethics, and reduce the application of immunosuppressive drugs. BMSCs is fitted the basic request of seed cells. On above all BMSCs transplantation will make the important help ofbrain injury.Basic on all above when MSCs co-culture with NSCs in vitro their fusion, proliferation and differentiation is important, the activation of endogenetic NSCs after MSCs transplantation to brain injury will make a significant theoretical contribution.Part one The experimental study of proliferation and differentiation when MSCs co-culture with NSCs in vitroObjectiveTo investigate the effects of the neural stem cell and marrow stromal cell's proliferation and differentiation when bone marrow stromal cell co-culture with neural stem cell together.Our experiment is to interpret the starting process of endogenetic NSCs and the theory of MSCs transfonnating into neuron to provide the theory help for clinical head injury therapy.MethodsRat neural stem cells were co-cultured with bone marrow stromal cells together in vitro with neural cells' conditioned medium, not serum. Proliferation's cells are without rat-tail's gum and differentiation's cells are with rat-tail's gum. The proportion of two cells is 1:0, 2:0, 10:1, 1:1, 1:10, 0:1 (NSC: MSC). The culture time is 3, 7, 14, 28 days. The number of neurons was obtained by using immunocytochemistry technique.ResultsInhibit the sphere growth of NSCs when NSCs co-culture with MSCs. The proportion of neurons to total cells was significantly increased by co-cultureneural stem cells with normal bone marrow stromal cells for two cells each other and enhance the growth of the neuron synaptic. They can enhance proliferation of neuron and maintain neuron.ConclusionThere are many good effects by neural stem cells co-culture with bone stromal cells, including differentiation, proliferation, the growth of neural synaptic and the maintain of neuron.Part two The study of MSCs' transplantation enhance the differentiation of endogenetic NSCs after rat brain injuryObjectiveWe translated the rat MSCs into surrounding injured district of rat brain by stereotaxically to understand the proliferation and differentiation of endogenetic NSCs and effect of rat neural motor function.MethodWe made Wistar rat brain injury model by free-falling bitting model. Adult male Wistar rats are divided randomly into 5 groups: 1st group, no transplantation and injury (normal control group, n=8), 2nd group, no transplantation into brain injured district after rat brain...