| Apoptosis is programmed cell death. When the machinery of apoptosis is improperly regulated, it will result in tumorigenesis and tumor progression. Survivin has recently been identified as a novel inhibitor of apoptosis (IAP). Unlike other members of the IAP family , survivin is characterized by a unique structure that contains a single baculovinis IAP repeat and no carboxyl-terminal RING finger motif. It is expressed in many common human cancers, but its expression is lost or down-regulated in normal adult tissues. Survivin blocks a common downstream part of two major apoptosis, the mitochondrial pathway and the death receptor pathway, by directly inhibiting terminal effector caspase-3 and caspase-7, and by interfering with caspase-9 activity. It also regulates the G2/M phase of the cell cycle by associating with mitotic spindle microtubules. Meanwhile, the mean apoptosis index(AI) in survivin-positive tumors significantly lower than the mean AI in survivin-negative tumors. Therefore , many scholars considered that survivin could suppress apoptosis and accelerated cell proliferation. Along with going deep into the research of survivin gene, survivin will become a new diagnosis and therapeutic target in tumor.Ovarian carcinoma is the most common cause of death among all gynecologic malignancies. The overall 5-year survival rate of patients is only about 40.9%. It heavily threaten the health of women. Nowadays, with the rapid development of molecular biology, it is generally accepted that the tumorigenesis and tumor progression are closely related to the changes of tumor cell biology. However, very little is known about the exact sequence of celluar and molecular events contributing to the development of ovarian malignancy. Many studies showed that survivin expression in tumors has been associated with increased aggressiveness and decreased patient survival in many kinds of cancer. Few reports have documented the quantitative and qualitative detections of survivin in ovarian carcinoma and benign ovarian tumor and the relationship between the expression ofsurvivin and clinicopathological features. We study the expression of survivin protein and mRNA in ovarian carcinoma, their relationship with proliferation activity of tumor cell measured by PCNA index and their clinical significance in ovarian carcinoma. Besides, there has been no report about antisense survivin(SWas) RNA inducing apoptosis of ovarian carcinoma in vitro and in vivo. It is unknown whether the antisense RNA technology may inhibit the expression of survivin and kill tumor cells or not. This study was designed to explore the effects of SWas on the growth of ovarian carcinoma cell lines and the human ovarian carcinoma transplanted subcutaneously in nude mice. To evaluate the anticancer effects of cisplatin, topotecan and cisplatin /topotecan to human ovarian epithelial cancer in nude mice. In addition, to investigate the changes in expression levels of survivin protein in ovarian epithelial cancer in nude mice during cisplatnu topotecan and cisplatin/topotecan treatment so to analyze the correlation between chemotherapy drugs and survivin expression. Methods1. The expression of survivin , PCNA protein and survivin mRNA in 38 cases of ovarian carcinoma, 12 cases of benign ovarian tumors were detected by immunnohisto-chemistry and in situ hybridization methods respectively. And the expression of survivin protein and mRNA in human ovarian carcinoma SK0V3 and SW626 cell lines were observed by immunnohistochemistry, immunnofluorescence and RT-PCR methods.2. The SWas eukaryotic vector pcDNA3-SWas by lipofectamine?000 was transferred into SKOV3 cells. The transfected cells were selected by G418, contrast with blank vector pcDNA3-neo transfected SKOV3 cells. Cells activity was investigated by MTT assay and the effect of SWas on cells growth was described by drawing its growth curve.3. Immunnohistochemistry and RT-PCR was performed to evaluate the inhibition effect of antisense survivin RNA on endogenous survivin protein and mRNA expres...
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