| To endeavor to obtain new resources with a sedative and sleep-enhancing activity as well asfor the rational use of the resources, we started an intensive research on the medicinal plants ofValeriana (MPV) in China. The MPV in eight provinces were comprehensively evaluatedconcerning pharmacognosy, chemotaxonomy and molecular biology in following aspects likebencaoological study, identification of the botanic origins, differentiation of the character of crudedrugs(CD), microscopic identification of CD, TCL analysis, HPLC fingerprint, hesperidinquantitative determination with HPLC and determination of ITS sequencing. The primaryresults are as follow: (1)Around 40 specimens of Valeriana were collected and 5 species were identified.18 samplesof Valeriana were collected from eight provinces in China, Germany and Japan. Characters of thebotanic origins, crude drugs and microscopic structures of 5 MPV were described and comparedwith each other, and index of Characters of the botanic origins, characters of CD, and microscopiccharacters of CD were also compiled. V. hardwickii Wall. was identified as focal point.Pharmacognosy studies showed that the form of rhizome and the basal aggregation leavies, thespreeding direction of inflorescences, the occurance of stone cells and hesperidin crystals weredifferent conspicuously among species. These could be used as identification characters. (2)Being made bencaoolgical researches, V.jatamansi Jones was described in the ancient bookson Chinese medicine only. It should be a traditional drug. (3)By means of TLC, the extraction of MPV with n-hexane, methanol, dichloromethane wereanalyzed. It was a quick, simple and convenient methed, and could be used for the evaluation ofthe quality of CD of Valeriana and differentiated CD of Valeriana .The TCL result showed thatthere were existence of chlorogenic acid and valtrate in the MPV. This probably had taxonomicsignificance. V. jatamansi Jones was similar to V. hardwickii Wall. in the methanol extract, andsimilar to V. officinalis L. collected from Germany in the dichloromethane extract. V. officinalis L.collected from Japan was different from other species. V. officinalis L. var. latifolia Miq. wassimilar to V.amurensis Smir. ex Komar. The differences of the chromatograms between different localities were smaller than thedifferences between the species. V. jatamansi Jones collected from ChenDu, KunMing, GuiYangand EmeiShan, characterized by the occurrence of one spot (Rf=0.88), were different from V.jatamansi Jones collected from AnGuo, YaAn, ShanXi. (4)The constituents of volatile oil of the MPV were undertaken by GC/MS combinationtechnology. The results showed that the common compounds were Bornyl acetate, Camphene,(-)-Spathulenol, Bicyclo[2.2.1]heptan-2-ol,1,7,7-trimethyl-,(1S-endo) and Benzene,1-methoxy-4-methyl-2-(1-methylethyl). The results had probably taxonomic significance. According to thecluster analysis, the MPV could be divided into three groups: Group A comprised V. jatamansiJones collected from different localities, Group B included V. officinalis L. var. latifolia Miq. ,V.amurensis Smir. ex Komar, V. officinalis L. collected from China, V. hardwickii Wall. collected fromdifferent localities, Group C included V. officinalis L. collected from Germany and Japan. V.officinalis L. var. latifolia Miq. was similar to V.amurensis Smir. ex Komar., V. jatamansi Jones wassimilar to V. officinalis L. collected from Germany and Japan. V. hardwickii Wall. could be dividedin the group of V. officinalis L. collected from China and V. officinalis L. var. latifolia Miq.. This IIIwas different from the results of pharmacognosy, TCL and HPLC fingerprint. (5) The HPLC/UV assay was used to establish the fingerprint of the hydrophilic constituents ofValeriana medicinal plants. The HPLC fingerprint of the lipophilic and hydrophilic constituents ofValeriana medicinal plants were established. HPLC fin...
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