| In this study, we use polyethersulfone(PES) as basic material to develop a biomaterials ,which can make artificial organs and equipments such as artificial heart,dialyzer and so no. The main research target is to study the process and technics of sulfonated, aminated and heparinized , biocompatibility and material's mechanical property.The principles and methods of organic synthesis were applied for heparinizating PES wholly. After sulfonated, 2-Morpholinoethanesulfoni acid monohydrate and N-(3-Dimethylaminopropyl)-N'-ethylcarbodiimidhydrochlorid served as condensation reagent ,1,30-Hexanediamine was connected noto PES. And then 1,30-Hexanediamine served as space arm, 2-Morpholinoethanesulfoni acid monohydrate and N-(3-Dimethylaminopropyl)-N'-ethylcarbodiimidhydrochlorid as condensation reagent too,thus heparin was linked to the PES materials modified by sulfonation via covalent bond . Because each benzene in PES molecule connects a sulfonyl(-so2-), which has the strong ability to attract electrons and deactivates the benzene.It is very difficult to sulfonate PES by electrophilic aromatic substitution reaction.Thus, sulfonated agent must have high reactivity and reation conditions affect on sulfonating process much.In our experiment, some factors such as reacting temperature, reacting time, stirring velocity and mole ratio of chlorosulfonic acid and PES, had been studied. The real reaction indicated that it is more suitable for sulfonating reaction in low temperature, dissolved by vitriol would make reacting system more symmetrical, controlling the relation of the sulfonated degree and the mole ratio of chlorosulfonic acid and PES it's likely to control the mole ratio to get proper products. Polyethersulfone (PES) was activated by chlorosulfonic acid as sulfonated agent in 10℃ reacting temperature, 2 hours reacting time and 800rpm stirring rate. After that, 2-Morpholinoethanesulfoni acid monohydrate and N-(3-Dimethylaminopropyl)-N'-ethylcarbodiimidhydrochlorid served as condensation reagent , in 300rpm, 25℃ reacting temperature, 3 hours, 1,30-Hexanediamine was connected noto PES. Because sulfonated process, aminated process and heparinized process are consecutive and complex courses.For studying these 3 process, we designed a perpendicular experiment. It indecates that interaction of sulfonated process and aminated process and different hexanediamine affected the whole process the most greatly. The best processing condition is that: sulfonated degree is 10%, hexanediamine molecular weight is 200 and amido mole ratio is 1:1 in heparinized process.For proving above 3 process had been made, we used ultraviolet spectroscopy analysis, scan electron microscope and fluorescence analysis to characterize. In fluorescence analysis,diazonium salt was connect onto sulfonated PES surface. And then through fluorescence microscope, we foung that sulfonic group is well-proportioned. It is very important to using this method to guide the sulfonated process. By ultraviolet spectroscopy analysis, it showed that experimental result is concurrent with origenal synthesis route. After sulfonated process, aminated process and heparinized process, heparin keeped initial structure and activity didn't changed.In coloring determination with toluidine quantitatively, the heparin content was 58.365mg/g.According suface caculation,the heparin content was 5.8mg/cm2.It reach the standard of heparinized anticoagulate biomaterials.It's important to evaluate biocompatibility of materials, because heparinized PES will contact with blood. In our research, we choose PEU, PES, GAMBRO and FRESENIUS hemodialysis vessel as contrast materials. When materials contact with blood, plasma protein adsorption is occured at first. Ingredient and structure of adsorbed layer deside the performance. In our experient, we found that improving surface hydrophilicity can decrease the quantity of plasma protein adsorption, increase the ratio of white protein in total plasma protein and reduce ball protein adsorption. But heparinized materials wer... |
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