| Signet ring cell carcinoma of gastrointestinal tract were observed ultrastructurally. And enzyme cytochemistry was applied to detect adenylate cyclase(ACL) activity of 56 cases of signet ring cell carcinoma. The results showed that there were three types of signet ring cell carcinoma: mucous type, microcapsular type and glycogenic type. In glycogen-type gastric cancer, the ACL activity in was obviously decreased. It is suggested that ACL activity deficiency may be responsible for glycogen accumulation in glycogen-type gastric cancer. Combined with mucin histochemical staining, we discussed the cellular origin of signet ring cell carcinoma. It was considered that signet ring cell carcinoma originates from the multipotential stem cells. And the ultrastructural morphological changes of cancer cells are the specific manifestations of their abnormal differentiation or their variant functional metabolism.30 cases of ovarian carcinoma with glycogen were studied. The result showed that the ages of such patients were younger when diagnosed, and distant metastasis and endometriosis appeared more in them. In comparison with ovarian carcinoma of common type, the survival rate decreased only in stage I of glycogen-rich type, but no difference in the other stages. The recurrence rate of glycogen-rich type is higher. The involvement of blood vessel was one of the signal for poor prognosis. In order to study serum sialyl SSEA-1 in patients with ovarian carcinoma, the serum levels of sialyl SSEA-1 antigen in 50 patients with ovarian carcinoma were detected byimmunoassay. The results showed that 50% patients had the levels of sialyl SSEA-1 antigen increased, especially in glycogen-rich carcinoma. The level of sialyl SSEA-1 antigen was associated with disease course and prognosis, sialyl SSEA-1 antigen might be an useful marker for monitoring of ovarian cancer. To study the association between glycogen-rich adenocarcinoma of the cervix and human papillos-18(HPV-18), ten cases of glycogen-rich cervical adenocarcinoma were analyzed by using polymerase chain reaction(PCR). The result showed that. 7 patients with glycogen-rich adenocarcinoma showed HPV-18 DNA positive. 8 patients have developed with recurrence and died of their cervical tumors. Glycogen-rich adenocarcinoma of the cervix is associated with HPV-18 aggressive clinical course. We also discussed the clinical characteristic of Glycogen-rich type endometrial cancers, relationship between glycogen-rich carcinoma and the expression of cancer-associated galactosyl-transferase.To study the relationship between MN/CA9 protein expresion and renal cell carcinoma. Immunohistochemisty and immunoblotting were used to detect the expresion of renal cell carcinoma, normal renal tissue and benign renal lesion. The result showed that expresion of MN/CA9 protein was found in renal cell carcinoma, especially in glycogen-rich type of renal cell carcinoma, but negative in normal renal tissue and cancer lesion. It is suggested that MN/CA9 may be a marker of glycogen-rich type of renal cell carcinoma. To study the frequent somatic mutations of von Hippel-Lindau tumor suppressor gene(VHL) and the expression of multiple drug resistence(mdr) gene in human renal carcinomas, 40 cases human renalcarcinomas were analyzed for VHL gene using polymerase chain reaction (PCR) and single strand conformational polymorphism analysis. 35 cases renal cell carcinomas were examined the product of mdr gene by immunohistochemical assay. Somatic mutations were detected in 63 percent( 19/30) glycogen-rich renal carcinomas including 13 deletion, 3 insertion. 2 missense and 1 nonsense mutation. The degree of mdr gene expression correlated with histopathlogic subtypes of renal carcinomas. Glycogen-rich renal carcinomas might be distingushed from other types by molecular genetic techniques.
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