| Atherosclerosis is considered to be the pathological basis of a variety of cardiovascular diseases.It is a vascular lesion characterized by chronic inflammation,and its development process includes oxidative stress,intimal damage,lipid infiltration,smooth muscle cell phenotypic transformation and inflammatory response.At present,percutaneous coronary intervention,as the main treatment for atherosclerosis,can quickly relieve acute symptoms such as myocardial ischemia,but many syndromes,such as new atherosclerosis,stent restenosis and other postoperative symptoms,seriously affect the prognosis of patients.In order to reduce the incidence of complications effectively,a variety of anticoagulants and lipid-lowering drugs are usually used in clinical practice for a long time.However,long-term use of the above drugs will lead to liver function damage and coagulation dysfunction,which will affect patients’ lives in the future.In 2007,Japanese scholar Shigeo Ohta pointed out that hydrogen can act as a selective antioxidant to reduce intracellular oxidative stress,thus alleviating the symptoms of stroke in rats,and the biological function of hydrogen was recognized.In the past ten years,hydrogen has shown good therapeutic effects in neurodegenerative diseases,metabolic syndrome,ischemic encephalopathy,autoimmune diseases and even a variety of adverse reactions caused by tumor radiotherapy and chemotherapy.Compared with the traditional treatment methods,hydrogen has a large therapeutic scope,high biological safety,and can play a good synergistic effect with a variety of drugs,so it has a strong prospect of clinical application.However,hydrogen is insoluble in water,and it is difficult to systematically study the cytological function of hydrogen by conventional means under laboratory conditions,which hinders the development of molecular hydrogen medicine.In order to solve the methodological difficulties existing in the experiment,this paper designed and manufactured a hydrogen-rich culture system to produce a medium circulation system which can provide stable hydrogen concentration for a long time.Compared with the traditional method of changing the hydrogen-rich medium repeatedly,the design of this paper solves the disadvantage of unstable hydrogen concentration and reduces the risk of bacterial contamination during the experiment.In this paper,a relatively good culture system has been obtained and applied via the exploration of gas composition.Through the above design,this paper further explored the application prospect of hydrogen in atherosclerosis.Based on endothelial cells,smooth muscle cells and macrophages in a variety of design and hydrogen cultivation system,it has been proved that the hydrogen can effectively promote endothelial cell lipid metabolic product and reduce oxidative stress level in the cells and the apoptosis at the same time by fluorescence microscope and flow cytometry and laser confocal microscope.It was proved that hydrogen could inhibit the transformation of smooth muscle phenotype to secrete induced by lipopolysaccharide and Western Blot.Enzyme linked immunosorbent assay showed that hydrogen could decrease the expression of interleukin-6 and tumor necrosis factor α in RAW 264.7 macrophages induced by hydrogen peroxide and inhibit the apoptosis.In this paper,after proving the effectiveness of hydrogen at the cytological level,carotid artery intima injury was carried out in New Zealand white rabbits,and high-fat feeding was performed to construct pathological areas of atherosclerosis.They then fed on hydrogen-rich water to explore the therapeutic effects of hydrogen on an animal level.Through section staining and immunohistochemistry,hydrogen can effectively reduce the intimal hyperplasia and the expression of inflammatory factors in the tissue.In summary,this paper aims to explore the therapeutic effect of hydrogen in atherosclerosis by designing a hydrogen-rich culture system,and to demonstrate the potential of hydrogen in the treatment of atherosclerosis at the zoological level. |
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