| Objectives: â‘ To establish a new canine model of focal cerebral infarction that be suit for the study of imaging diagnosis and thrombolytic therapy, and to evaluate the technique feasibility and stability; â‘¡ To investigate the suitable injection rate and dose of multislice CT perfusion imaging; â‘¢ To study the effect and complications of r-Sak for acute cerebral infarction thrombolysis with different doses, different way in canine mode, and compare with UK, and then to investigate the fit dose, the safety and the efficacy of intraarterial r-Sak ;â‘£ To observe the effect of r-Sak on plasma levels of coagulation and thrombolytic parameters in dogs.Methods: â‘ Six beagle dogs were selected into the study. The white thrombus was coagulated with dog's self-venous blood. The 4F headhounter catheter was inserted into the left internal carotid artery and the thrombi was injected under fluoroscopic guidance. The cerebral angiography was performed before and after the embolization. The patency of the occluded cerebral arteries were examined by angiography at 1, 2 and 5h after the embolization. These dogs were sacrificed and the cerebrum was taken out for pathology study at 24h; â‘¡ Randomizedblock design was adopted in this study. The treatment factor-contrast medUm injection rate was classified into 3, 4, 5, 6 and 8(ml/s); the subjects were divided into 5 blocks grouped by contrast medUm injection dose: 0.5, 0.75, 1.0, 1.25 and 1.5(ml/kg). After post processing, time to peak (TTP) and rise value (RV) were measured in superior sagital venous; â‘¢ The embolism model of acute cerebral infarction was established with interventional technique in 36 adult beagle dogs, which were randomly divided into control(saline, 10ml, IA), r-Sak low dose(r-Sak, 5,000U/kg, IA), r-Sak middle dose(r-Sak, 10,000U/kg, IA), r-Sak high dose(r-Sak, 20,000U/kg, IA), UK(UK, 10,000U/kg, IA) and intravenous r-Sak(r-Sak, 10,000U/kg, IV) groups. Angiography and thrombolysis within 30 minutes were performed 5 hours after embolization with different way, and angiography was repeated half, 1 and 2 hours after thrombolysis to observe recanalization. Blood samples were collected at a series of time pre- and post-thrombolysis to examine the plasma levels of PT, APTT and D-dimer. These canines were sacrificed, and their cerebri and vessels were taken out for pathologic study at 24 hours.Results: â‘ The middle cerebral artery occlusion occurred in all 6 dogs, 4 of which had other cerebral artery occlusion. All occluded arteries were not patent at 2h after embolization, but 1 posterior communicating artery was partly patent at 5h. All dogs were survived at 24h without any severe complications. The cerebral infarctions were found in dog's deepcerebrum; (D Injection rate had a statistical significant effect on TTP of superior sagital venous. There was no significant difference among group 5, 6 and 8ml/s. Compared with 8ml/s, TTP of superior sagital venous was prolonged when injection rate 3, 4 ml/s were adopted. Injection dose had a statistical significant effect on RV of superior sagital venous. There was no significant difference among group 1, 1.25 and 1.5ml/kg. Compared with 1..5ml/kg, RV of superior sagital venous was prolonged when injection dose 0.5,0.75 ml/kg were adopted (D The rate of efficacy in 2 hours after thrombolysis was 10.0% (1/10) in control group, 40.0% (4/10) in low dose group, 90.9% (10/11) in middle dose group, 100% (9/9)in high dose group, 33.3% (3/9) in UK group and 45.5%(4/11) in intravenous group, respectively. There statistically are obvious differences among groups(p<0.05). The rate of complete recanalization was 0(0/10), 10% (1/10), 36.4% (4/11), 66.7% (6/9), 0(0/9), 9.09%(1/11), respectively. There are also statistically obvious differences among groups(p<0.05);(4) The death occurred in 1 canine of high dose group whin 24 hours after thrombolysis, the others were survived. The hemorrhage was found in puncture point with 6, 2 dogs respectively of the high dose and middle dose group after thrombolysis. There were better clinical re...
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