Experimental Study On Preparation Of Artery Vascular Biomaterials And Procedure Of Artery Adhesive Anastomoses

Objective: To prepare artery vascular biomaterials and evaluate the feasibility of transplantation with decellularized artery allografts.Methods: (1)This study set up a new multi-step process to access the preparation of decellularized artery grafts by comparing the effects of lour extraction chemicals [t-octyl-phenoxypolyethoxyethanol (Triton X-100), tri(n-butyl)phosphate (TnBP), and sodium dodecyl sulfate (SDS) and trypsin] on thoracic artery vascular cellularity, structure and pulling mechanical natures in rabbits. With the multi-step process, acellular rabbit artry, vein and rat small-intestine submucosa were produced. (2)Chitosan membranes were fabricated by casting method adding glycerol plastical agent The mechanical properties and biocompatibility to smooth muscle cells were evaluated. (3)The fescia biomaterials, and artery vessel treated by frozen-drying and glutaraldehyde were produced The mechanical properties and viscoelasticity of all the above biomaterials were assessed. (4) And fee decellularized carotid artery allografts were transplanted mother rabbits.Results: (1)A multi-step process could remove all cells in the rabbit artery. Acellular artery, vein and rat small-intestine submucosa were produced with me extracellular matrix well conserved. The pulling mechanical properties of decellularized carotid artery were similar to the control (p>0.05). There are few immunogenicity and cytotoxicity in the acellular artery matrix. (2) The Chitosan films of 2% concentration with 2% glycerine was good in mechanical properties and biocompatibility to cells.(3)The viscoelasticity of glutaraldehyde-treated vessel materials was deficit(4)And the decellularized carotid artery allografts(diameter of 2mm) were patent at transplanting up to 2 months.Conclusion: The acellular artery vascular biomaterials matrix is well prepared with multi-step process including detergents, such as TritonX-100 SDS without compromising me graft structure or mechanical properties significently. The Chitosan films was suitable to be artery materials in mechanical properties and biocompatibility to cells. And the mechanical properties and viscoelasticity of all the above biomaterials are good except the glutaraldehyde-treated. And the carotid artery allografts(diameter of 2mm) decellularized by the process are patent at transplanting up to 2 months.