Electrical Stimulation To The Hippocampus Of Rats To Found Animal Models And Detection The Expression Of Gst Gene With Gene Cmos Chip Technique In Intractable Epilepsy

Background: Epilepsy (EP) is a kind of common disease in Pediatrics and Neurology departments but the pathogenesis of EP is not well known especially that of the Intractable Epilepsy (IE) to which all kinds of anti-epilepsy drugs (AEDs) are noneffective. Selection appropriate animal models is the right way to study the pathogenesis and treatment of EP. There have been tens of animal models to study EP till now and celerity-kindled-rats model is thought as the most practical animal model with which we can simulate human complex partial epilepsy. The main EEG character is after-discharge high-amplitude pike wave or multi-pike wave epileptiform encephalic electrical activity which appears after the ending of stimulating. The overseas study showed kindled-rats respond differently to phenitoin (PHT), penobarbital (PB) and other classical AEDs and the drug-resistant kindled-rats could be screened out with the index of after-discharge threshold. Our examination has established the celerity-electricity-kindled-rats IE model with PHT .There are 20-30% EP are IE. Why some EP respond to drugs but others not? Present study shows that relates to the expression of multidrug resistancegene(MDR).MDR codes a kind of P-glycoprotein(P-gp) which pumps PHT or other AEDs out of cells by energy-dependent pumps. The increased expression of MDR is found in the EP patients brains specimen. But the treatment doesn't work which reverses the expression of MDR. Eberts' study showed that gene mechanism may take part in the formation of drug-resistance rats. So we can find out the correlative gene to expatiatethe drug-resistance mechanism.The possible mechanism being used to explaining IE include drug-resistance gene, change of synapse plasticity, recombination of nerve net and disorder resulting from gene mutation. Gene CMOS chip is a kind of laboratory technique in which a group of genes are integrated on a piece of glass. Two kinds of probes which come from two different tissues or cells are made with two kinds of fluorescence dying matter . The probes are mixed and hybridized with gene CMOS chip and washed, scanned with special fluorescence waves and then we can get the expression spectrum difference of this group of genes in these two tissues. So gene CMOS chip technique is a effective tool to investigate the relation between disease and gene. In our examination we detect the gene expression spectrum difference between PHT resistance EP rats and PHT effective EP rats with gene CMOS technique and at the same time we detect the GST(glutathione-S-transferase) gene in EP patients brains and blood with gene CMOS chip technique and immunohistochemistry technique.Objective: Trough the founding of kingding model in three different ways: unilateral kinding , bilateral kinging and alternate-site kinding we disstuss the possible reasons between the different kinding models and the possible mechanism of epileptic activity. To investigate the gene mechanism of drug-resistance in IE and the expression of GST gene in IE patients brains.Methods: 60 adults Wistar rats were divided into 3 groups:UK BK and AK groups randomly. According to Goddard's method. We implanted the electrode to the hippocampus(one in UA group;two in BK and AK groups).After 7days the rats received electrical stimulations daily once:400 uA ,60Hz,lms. we record the kinding rate and the stage of convulsions.To screen out 7 drug-resistance rats and 6 drug effective rats by using PHT and electricity stimulating amygdala kindled-rats model with Loscher method. Then to assay the gene expression in two kinds of rats cDNA...