Study On The Role Of CC-chemokine Receptor 5 In Associated Diseases

We aim to study on the role of CCR5 in type 1 diabetes mellitus and invasive ductal breast carcinoma. In part 1 we explored the role of CCR5 in type 1 diabetes mellitus by RNAi and FACS in T cells and model animals. Our resultsshowed thatCCR5 may play a role in type 1 diabetes by affecting Th1/Th2 cytokines balance and control progress in early insulitis in NOD scid mice. In part 2 we investigated the expression of CCR5 in invasive ductal breast carcinoma by immunohistochemical stain and MIP-1α , MIP-1β and RANTES concentrations were measured by ELISA in the serums of associated patients. The result showed that The expression of CCR5 was detected in invasive ductal breast carcinoma and RANTES concentrations in the serum of breast carcinoma patients increased significantly. In part 3 the encoding sequence of human ProTα was inserted into the prokaryotical expression vector pBV220. The expression of protein ProTα was induced by heat. The biological activity of rh ProTα was assayed using T—cell E—rosette formation test.. It could increase the E—rosette formationrate of human peripheral mononuclear cells.