Study On Quality Evaluation On Herbal Drug Culticated By Standard Operation Procedure (Ⅷ)

To meet the need of the quality control during the producing process of traditional Chinese drugs and quality evaluation of medicinal materials, key and general technology during the producing process of cultivated Schisandra chinensis were elucidated. Based on that, chemical and biological evaluating methods were established. Systemic evaluation and comparative study between variety and cultivated and that of wild Schisandra chinensis have been carried out either, which lay a foundation for compositing the SOP of cultivating procedure of S.chinensis Baill.First, a HPLC method was established to simultaneously determine the content of lignans (i.e Deoxyschizandrin, y-Schisandrin and Schisandrin et.).Then it was used to analyze the three lignans of S.chinensis Baill of different collecting time and various drying methods from different producing areas, respectively. It showed that the content of the three active ingredients of S.chinensis(Turcz.)Baill increased with the seasons during the period of August and October. Since the variation of the sum of three lignans could express the basic variation of effective ingredients, it can be used to ascertain the collecting time. It proved that all the three active ingredients collected in October after fruit ripen and frost so that it can be considered as the optimum collecting time. Different drying methods indicated that the content of the active ingredients from the samples dried in the oven at 50°C and in the shade were higher than those dried by other methods. It was statistically tested that there was no significant differences. It can be considered that Schisandra chinensis with high content of active ingredients and good appearance can be obtained both by drying in the oven at 50℃ and in the shade. Dried in the oven at too high temperature will cause more dry sample loss. Furthermore, appearances are not very good. Therefore, temperature should be strictly controlled and high cost would be needed when we take oven drying method. Contrary, drying in the shade will cost less and have better appearance but need turning over more frequently for lest it will go moldy.