Central China Schisandra Fruit Hplc Fingerprint Of Research

Schisandra sphenanthera Rehd. et Wils. (S. sphenanthera) is a kind of perennial lianas which belongs to Schisandra Michx. in Schisandraceae. Its'dried ripening fruits are called nan-wu-wei-zi. The fruit are rich in lignans, which have lowering SGPT, protect the central nervous system, anti-aging, anti-tumor effect.In this study, to a larger range of the 18 national central origin for the study S. sphenanthera, we optimized the extraction technology of lignans using ultrasonic extraction,analyzed different origin S. sphenanthera by four lignans such as schisandrin, schisantherin A, deoxyschizandrin, y-schizandrin, built a suitable HPLC condition, finally built the chromatography fingerprints of S. sphenanthera by HPLC and studied by chemical pattern recognition methods. With a view to S. sphenanthera by scientifically, objectively, equitably and effective quality control and standardization to achieve the quality of scientific basis of Chinese medicine.The results are as follows:1. Ultrasonic technique was applied in extraction lignans from Schisandra sphenanthera Rehd. et Wils. Various factors was studied by orthogonal experiment. The results show that in schisandrin different factors affecting the primary and secondary order is rate of material to solvent>solvent>ultrasonic treating time>extraction times, in schisantherin A, deoxyschizandrin, y-schizandrin, different factors affecting the primary and secondary order is rate of material to solvent>solvent>extraction times>ultrasonic treating time. However, the result of various factors had no significant effect. The optimal extraction condition:trichloromethane as solvent, rate of material to solvent 1:5, ultrasonic treating time 30min, extracting once. This method was simple, effective and feasible, and can be used to control the quality of Schisandra sphenanthera Rehd. et Wils.2. The lignans kind and the lignans qualities were vary in different origin. The results show that schisantherin A contents from all of the 18 origins S. sphenanthera were greater than 0.12%. In Xiuwu, Lushi of Henan province, Zhashui fengzhen area, Zhashui yingpan area, Huaxian area of Sh'province, were the better place of origin which the contents of schisantherin A were greater than 0.670%. The contents of schisandrin were lower, most of 0.006%～0.023% in the range, except Zhouqu in Gansu province was 0.605%. In Xiuwu of Henan province, Fengzhen, Yingpan, Huaxian of Sh'province, the contents of schisantherin A and deoxyschizandrin were higher, most of 0.688%～0.910% and 0.583%～1.013%, the contents ofγ-schizandrin were lower, most of 0.116 %～0.542%. The plant was mainly distributed in southern and eastern area of Qinling and the southern area of Taihangshan; In Qingchuan of Sichuan province, Wuxi of Chongqing area, Zhouqu of Gansu province, the contents of schisantherin A and deoxyschizandrin were lower, most of 0.289 % or 0.334%, the contents ofγ-schizandrin were higher, most of 0.874%～1.489%. The plant was mainly distributed in the western area of Qinling and Sichuan basin.3. A rapid and convenient HPLC analysis method had been developed for chromatography fingerprints of S. sphenanthera. The HPLC system consisted Shimadzu LC-2010(Shimadzu Corp, Kyoto, Japan) and Shimadzu LC solution software. Separation was achieved on a Shimadzu C18 column (Phenomenex,150×4.6 mm; 5μm particle size). Mobile phase A:water, B:methanol, gradient elution was with 0～20 min,45%～65%B; 20～45 min,65%～75%B; 45～50 min,75%B; 50～70 min,75%～90%B; 70～100 min,90%～100%B; The flow rate was adjusted to 0.8 mL/min, the detection wavelength to 280 nm, recording time was 100 min, and 8μL of sample was injected. All separations were performed at 30℃. After the checking of the linearity, the accurately, the repeatability and the precision, the method was showed suitable for the chromatography fingerprints of Schisandra sphenanthera Rehd. et Wils.4. Constructed chromatography fingerprints of Schisandra sphenanthera Rehd. et Wils. from Shaanxi, Gansu, Henan, Shanxi, Anhui, Zhejiang, Sichuan, Chongqing provinces and cities of 18 different origins. As schisantherin A to mark peak,13 common peaks were confirmed. The relative retention time were more stable, the relative peak area had great differences, indicating different origin S. sphenanthera. lignans there were some differences. The similarity of origin fingerprints found in the place of Lushi of Henan province, Lin'an of Zhejiang province, Zhen'an of Sh'province were lower, and other origins the similarity were more than 0.790, indicating that this fingerprint were characteristic, for the identification of different origins to provide a central reference of S. sphenanthera. Fingerprint data and lignans determination results show that in Xiuwu of Henan province, Yingpan, Fengzhen, Xunyang, Huaxian of Sh'province, the quality were much better than other origins of S. sphenanthera. This identified 13 strong peaks for the 18 batches of samples by the total, and the total peak area ratio of the total peak area greater as fingerprint peaks show that the fingerprint of S. sphenanthera. has similarity and specificity, and may constitute the unique fingerprint of S. sphenanthera.5.The chemical data obtained from the fingerprints were studied with several chemical pattern-recognition methods. Cluster analysis, PCA, and stepwise discrimination analysis were used to analyze the lignans components fingerprints. Cluster analysis results divided the origin into four categories. And stepwise discrimination analysis was used to make a discriminate function to identify the habitats of S. sphenanthera. The correct rate of habitat identification was 88.9%. This method can be used to control the quality of Schisandra sphenanthera Rehd. et Wils.