| [Objective] Intrahepatic cholestasis of pregnancy (ICP) is one kind of common and idiopathic complications of pregnancy which occurs during second or third trimester and severely threatens the life of perinatal babies. Though studies suggest that the disturbance of maternal immune system function may relate to the pathogenesis of the disease, its etiology and pathogenesis are unknown. Recent research on maternal-fetal interface has suggested the decidual tissue plays an integral role in normal pregnancy and certain gestational diseases. So we investigate human decidual function in normal pregnancy and ICP.[Methods] We measure the percentage of human decidual lymphocyte subpopulations: CD4+, CD8+ and CD56+ by flowcytometry in normal pregnancy and ICP. And we determine the concentrations of IL-10, IFN-γ IL-8 and RANTES spontaneously secreted by cultured human decidual stromal cells (DSC) in normal pregnancy and ICP using the method of Elisa.[Results] 1. Normal late pregnancy was characterized by the increased percentage of CD4+ and CD8+, the decreased percentage of CD56+ and the higher RANTES secreted by human decidual stromal cells compared tonormal early pregnancy (P<0.05). 2. ICP was characterized by the increased ratio of CD4+/CDg+, the increased percentage of CDs6+ and the higher IFN-y, IL-8, RANTES and the lower IL-10 and IL-10/IFN-y secreted by human decidual stromal cells compared to normal late pregnancy (P<0.05). 3. Dexamethasone of 1.0umol/l significantly increased the concentration of IL-10 and decreased the concentration of IFN-y, IL-8, RANTES secreted by human decidual stromal cells in ICP (P<0.05).[Conclusion] Human decidual lymphocyte subpopulations and cytokines spontaneously secreted by decidual stromal cells alter with the different stages of normal pregnancy and ICP. It suggests the decidual function may play an important role in normal pregnancy and ICP. Dexamethasone can improve the prognosis of ICP probably through changing the cytokines secreted by DSC.
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