| Objective WO-1 bio-derived bone scaffold materials were made. These ways were studied, such as physical and chemical property, controlled release property, cellular compatibility, bacteriostasis ability and the effect of repairing bone defect. Methods Bio-derived bone scaffold materials, collagen loaded bio-derived bone scaffold materials, WO-1 loaded bio-derived bone scaffold materials were maded by using fresh bone, type-I collagen and WO-1 in a series of physical and chemical methods. (1) Physical and chemical property of three bio-derived bone scaffold materials: The color, size and dimensional frame were observed, the change of the materials obtained by different ways were preliminary known; the micro-dimension change was observed; the pore size and pore ratio were tested by electronic scanning microscope; the constitute components were analysed by X-ray diffraction; the mechanical property was tested. (2) Cellular compatibility of three bio-derived bone scaffold materials: The experiment was divided into group A, B, C according to different materials.Group A: Osteoblasts were cultured with bio-derived bone scaffold materials; Group B: Osteoblasts were cultured with collagen loaded bio-derived bone scaffold materials; Group C: Osteoblasts were cultured with WO-1 loaded bio-derived bone scaffold materials. Osteoblasts in 1 × 107/ml density were cultured with the three materials for 1 > 3 * 5 ^ 7 days. The adhesion and growth of osteoblasts were observed by phase microscope and electronic scanning microscope, the cell viability and ALP were examined, the cell cycle was analysed by flow cytometer. ? The release kinetics of WO-1 from WO-1 bio-derived bone scaffold materials: WO-1 loaded bio-derived bone scaffold materials was made by 3H-tetracycline absorbed to collagen bio -derived bone scaffold materials, the release rate of 3H-tetracycline from WO-1 bio-derived scaffold materials was counted for K 2% 4> 6^ 8 days by Gama device. ? The antifection property of WO-1 bio-derived bone scaffold materials: WO-1 bio-derived bone scaffold materials was experiment group, collagen bio-derived bone scaffold materials and bio-derived bone scaffold materials as control group. Staphylococcus autreus> escherichia coli and aeruginosus bacillus were cultured with three materials in nutrient medium, cultured again and again, the antifection effect of the materials was observed. ? The effect of repairing bone defect of the different bio-derived bone scaffold materials: Newzeland white rabbit 96 were choosed, and the critical size defect were made for 1.5mm long. The experiment animals was divided into An B> CU D group according to different transplant materials. Group A: Bio-derived bone scaffold materials; Group B: Collagen bio-derived bone scaffold materials; Group C: WO-1 bio-derived bone scaffold materials; Group D: The complex materials of WO-1bio-derived bone scaffold materials cultured with osteoblasts. The experiment animal were killed at 6> 12 weeks postoperatively, a series of examination were carried out, including X-ray observation^ histomorphological analysis > immunohistochemical detection of type-I collagem the content of calcium and phosphate, the mechnical strong detection. 40 Newzeland white rabbit were choosed, WO-1 bio-derived bone scaffold materials were grafted the critical size defect of raddi. The vein blood were obtained from rabbit ear for 1% 3> 5> 7, 9> 12% -14% 2K 28 days, the concentration of WO-1 in blood was detected, and two animal were killed for 7> 9% 16% 23% 30 days, the muscles and bone around graft were obtained for about 500-700g, the content of WO-1 of the muscle and bone were detected. Results: ? physical and chemical property of the three bio-derived bone scaffold materials: Bio-derived bone scaffold materials appear white, collagen bio-derived bone scaffold materials appear white, WO-1 bio-derived bone scaffold materials appear light yellow; The size and volume of three materials were not changed, the natural network system was maintained; The surface of pore of bio-derived bone scaffold materials was smooth; The surface of the other materials was gross, the silk network was observed; There was no difference in pore size and pore ratio of the three materials by electronic scanning microscope (P>0.05 ) ; There were no difference in content and constitute components of the three materials (P>0.05). ? Cellular compatibility of the three bio-derived bone scaffold materials: Osteoblasts were adhered to three materials well, and grow% differentiate and proliferate in pore of materials. The adhesion ratio was A |
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