The Effect Of Oral Mucosa With Transfected IFN-γ Gene Implantation On Denuded Hard Palate To The Growth Of Maxilla In Yong Rats

Objective : To explore The biological activities of interferon- Y on fibroblast from rat palatal scar. To explore method of isolation and culture of rat palate mucosa epidermal stem cells(RPMESC) in vitro. To observe the effect of oral mucosa with transfected IFN- Y gene implantation on denuded hard palate to the growth of maxilla in yong rats. Methods: found out the effects of recombinant IFN- Y on the the growth, collagen biosynthesis of fibroblasts and the contraction of fibroblast-populated collagen lattices from palatal scar tissue with the method of "three dimensional" cell culture system. RFPMESC were isolated by means of collagen-fibronectin coated culture flask rapid adhering method. The cell culture mediumwere prepared with Ca²⁺-free EMEM mixed 50% fibroblast conditioned medium. The cultured cells were identified by immunohistochemistry staining of integrin-pi and keratin-15. The IFN-γ gene was transfected into mucosa epidermal stem cells in vitro , the expression level and activity of IFN- Y in the cultured supernatant liquid of mucosa epithelial cell was investigated, denuded hard palate were repaired by amnia and IFN-γ transfected oral mucous epidermal stem cells vegetating on amnia. The maxillary growth was investigated as growth of mouse stop. The histological change was observed with HE staining and sirus staining and electron microscope. Results: The results showed that IFN-γ not only could reduce the growth and collagen synthesis of palatal scar-derived fibroblasts, but also inhibit the contraction of fibroblast-populated Collagen Lattices. RFPMESC can grow well in the epidermal stem cells medium and the cultured cells were strongly positive with immunohistochemistry staining of integrin-β1 and keratin-15.The cell transfected IFN- Y gene can secrete active IFN- y . The cultured supernatant liquid of mucosa epithelial cell can affect the growth and proliferation. It played the proportion of type I / III collagen and hard palate asymmetry ratio down that amnia and IFN- Y transfected oral mucous epidermal stem cells vegetating on amnia repaired denuded hard palate. Conclusion: According to above results ,authors think IFN- Y may is a negative regulated cytokine to palatal scar formation and wound healing, and it can play an important role in the prevention and treatment of palatal scars. RFPMESC could be isolated and cultured in vitro by means of collagen-fibronectin coated culture flask rapid adhering method and the medium mixed from Ca2 + -free EMEM and 50% fibroblast conditioned medium. The cell can be transfected IFN- Y gene and secrete active IFN- Y . Amnia and IFN- Y transfected oral mucous epidermal stem cells vegetating on amnia play a positive action for prevention of secondary maxillary deformity, oral mucous epidermal stem cellsvegetating on amnia excelles amnia, the IFN- Y transfected excel slightly not transfected, but latter two group lack statistical difference.Based on these findigs, we think it is very helpful for prevention of secondary maxillary deformity that amnia and IFN-Y transfected oral mucous epidermal stem cells vegetating on amnia repair denuded hard palate, but the effect fasion of IFN-Y for prevention of secondary maxillary deformity await studying.